"ARFGAP1" Related Products


Recombinant Human ARFGAP1 293 Cell Lysate

Cat.No.: ARFGAP1-8754HCL
Description: Antigen standard for ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ARFGAP1 ADP-ribosylation factor GTPase activating protein 1 [ Homo sapiens ]
Official Symbol: ARFGAP1
Synonyms: ARFGAP1; ADP-ribosylation factor GTPase activating protein 1; ADP ribosylation factor 1 GTPase activating protein , ARF1GAP; ADP-ribosylation factor GTPase-activating protein 1; bA261N11.3; FLJ10767; ARF1 GAP; ARF GAP 1; GAP protein; ARF1-directed GTPase-activating protein; ADP-ribosylation factor 1 GTPase activating protein; ADP-ribosylation factor 1 GTPase-activating protein; ARF1GAP; HRIHFB2281; MGC39924;
Gene ID: 55738
mRNA Refseq: NM_175609
Protein Refseq: NP_783202
MIM: 608377
UniProt ID: Q8N6T3
Chromosome Location: 20q13.33
Pathway: Activation of Chaperone Genes by XBP1(S), organism-specific biosystem; Activation of Chaperones by IRE1alpha, organism-specific biosystem; Arf1 pathway, organism-specific biosystem; COPI Mediated Transport, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Endocytosis, organism-specific biosystem;
Function: ARF GTPase activator activity; metal ion binding; zinc ion binding;

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