Recombinant Human AQP1 293 Cell Lysate

• Cat.No.: AQP1-8770HCL

Specification

• Description: Antigen standard for aquaporin 1 (Colton blood group) (AQP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AQP1 aquaporin 1 (Colton blood group) [ Homo sapiens ]
• Official Symbol: AQP1
• Synonyms: AQP1; aquaporin 1 (Colton blood group); aquaporin 1 (channel forming integral protein, 28kDa) , aquaporin 1 (channel forming integral protein, 28kDa, CO blood group) , CO, Colton blood group; aquaporin-1; CHIP28; aquaporin-CHIP; Colton blood group; urine water channel; channel-like integral membrane protein, 28-kDa; water channel protein for red blood cells and kidney proximal tubule; aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group); CO; AQP-CHIP; MGC26324
• Gene ID: 358
• mRNA Refseq: NM_001185060
• Protein Refseq: NP_001171989
• MIM: 107776
• UniProt ID: P29972
• Chromosome Location: 7p14
• Pathway: Aquaporin-mediated transport, organism-specific biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Passive Transport by Aquaporins, organism-specific biosystem; Proximal tubule bicarbonate reclamation, organism-specific biosystem; Proximal tubule bicarbonate reclamation, conserved biosystem; Regulation of Water Balance by Renal Aquaporins, organism-specific biosystem
• Function: ammonia transmembrane transporter activity; carbon dioxide transmembrane transporter activity; glycerol transmembrane transporter activity; intracellular cGMP activated cation channel activity; nitric oxide transmembrane transporter activity; potassium channel activity; potassium ion transmembrane transporter activity; protein binding; transmembrane transporter activity; water channel activity; water channel activity; water channel activity; water transmembrane transporter activity