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Recombinant Human AP3S2 293 Cell Lysate

Cat.No. : AP3S2-8807HCL
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  • Gene Information
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Description : Antigen standard for adaptor-related protein complex 3, sigma 2 subunit (AP3S2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AP3S2 adaptor-related protein complex 3, sigma 2 subunit [ Homo sapiens ]
Official Symbol : AP3S2
Synonyms : AP3S2; adaptor-related protein complex 3, sigma 2 subunit; AP-3 complex subunit sigma-2; sigma3b; sigma-3B-adaptin; sigma-adaptin 3b; adaptor complex sigma3B; AP-3 complex subunit sigma-3B; clathrin-associated/assembly/adaptor protein, small 4, 22-kD; AP3S3; FLJ35955;
Gene ID : 10239
mRNA Refseq : NM_005829
Protein Refseq : NP_005820
MIM : 602416
UniProt ID : P59780
Chromosome Location : 15q26.1
Pathway : Lysosome, organism-specific biosystem; Lysosome, conserved biosystem;
Function : protein transporter activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (16)

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In addition to cancer treatment, what other areas can AP3S2 be used? 12/13/2022

AP3S2 can also be used in the treatment of inflammation, autoimmune diseases and other fields.

What are the physicochemical properties of AP3S2 ? 05/20/2022

The AP3S2 has a relative molecular mass of about 16-18KDa and an isoelectric point of about 5.5-6.0.

What are the structural characteristics of AP3S2 ? 04/22/2022

The AP3S2 belongs to the small G protein family and has the structure of GTPase activity, which is similar to other small G proteins in terms of molecular structure.

What are the possible future application prospects of AP3S2 ? 11/17/2021

With the continuous research and in-depth understanding of the mechanism of action of AP3S2 , its future treatment prospects in tumor, inflammation and other fields are broad.

Can AP3S2 be used in clinical treatment? 10/16/2021

At present, there are no reports and studies on the application of AP3S2 in clinical treatment.

Which diseases is the of AP3S2 valuable for research and treatment? 09/26/2021

At present, the therapeutic value of recombinant AP3S2 protein is mainly concentrated in the fields of tumor and inflammation.

Why was recombinant AP3S2 chosen for cancer treatment? 08/06/2021

AP3S2 is involved in intracellular protein transport, which can directly affect the growth and proliferation of cancer cells. It can also promote the immune system to clear cancer cells.

Can the combination of AP3S2 and other therapeutic agents have a better therapeutic effect? 05/10/2021

The combination of AP3S2 and other therapeutic agents can have a better therapeutic effect, but the specific effect needs to be further studied.

What are the best storage conditions for AP3S2 ? 05/03/2021

The optimal storage condition of AP3S2 is the refrigerator at -20℃, avoid light and moisturize, and do not repeatedly freeze and thaw.

What is the relationship between AP3S2 and immune system? 03/28/2021

Recombinant AP3S2 protein is involved in intracellular immune regulation and can affect the tubule system, cytophagocytosis and the formation of acidic lysosomes.

What is the biological function of AP3S2 ? 09/13/2020

The AP3S2 is involved in protein transport from the Golgi apparatus to the endoplasmic reticulum during intracellular transport.

What is the clinical manifestation of recombinant AP3S2 in mouse models? 04/23/2020

Recombinant AP3S2 protein in mouse model can significantly promote tumor growth inhibition and reduce tumor metastasis.

What is the cost of synthesizing AP3S2 ? 03/18/2020

The cost of synthesis of AP3S2 varies according to different manufacturers, methods and other factors.

What is the progress of preclinical research on AP3S2 ? 01/19/2020

At present, the preclinical research of AP3S2 mainly focuses on in vivo and in vitro experiments and animal model studies.

What is the synthesis method of AP3S2 ? 10/26/2019

AP3S2 can be prepared by synthesis, expression and purification.

How difficult is the preparation of AP3S2 ? 10/24/2019

The preparation difficulty of AP3S2 depends on the specific preparation method and experimental conditions.

Customer Reviews (4)

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Reviews
11/11/2020

    Green production process of AP3S2 is very advanced, using the most environmentally friendly production process and is very friendly to the environment.

    09/11/2020

      They have a wide range of products and can always find the AP3S2 we need.

      09/02/2020

        Catalytic activity of AP3S2 is really amazing, and it can bring unexpected catalytic effects every time it is used, and its performance is impressive.

        08/10/2020

          The reproducibility of AP3S2 can be said to be almost 100 percent, and the results are very consistent in every experiment or application, which is an important indication of its excellent quality.

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