Recombinant Human AP3S2 293 Cell Lysate
Cat.No. : | AP3S2-8807HCL |
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- Gene Information
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Description : | Antigen standard for adaptor-related protein complex 3, sigma 2 subunit (AP3S2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AP3S2 adaptor-related protein complex 3, sigma 2 subunit [ Homo sapiens ] |
Official Symbol : | AP3S2 |
Synonyms : | AP3S2; adaptor-related protein complex 3, sigma 2 subunit; AP-3 complex subunit sigma-2; sigma3b; sigma-3B-adaptin; sigma-adaptin 3b; adaptor complex sigma3B; AP-3 complex subunit sigma-3B; clathrin-associated/assembly/adaptor protein, small 4, 22-kD; AP3S3; FLJ35955; |
Gene ID : | 10239 |
mRNA Refseq : | NM_005829 |
Protein Refseq : | NP_005820 |
MIM : | 602416 |
UniProt ID : | P59780 |
Chromosome Location : | 15q26.1 |
Pathway : | Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; |
Function : | protein transporter activity; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (16)
Ask a questionAP3S2 can also be used in the treatment of inflammation, autoimmune diseases and other fields.
The AP3S2 has a relative molecular mass of about 16-18KDa and an isoelectric point of about 5.5-6.0.
The AP3S2 belongs to the small G protein family and has the structure of GTPase activity, which is similar to other small G proteins in terms of molecular structure.
With the continuous research and in-depth understanding of the mechanism of action of AP3S2 , its future treatment prospects in tumor, inflammation and other fields are broad.
At present, there are no reports and studies on the application of AP3S2 in clinical treatment.
At present, the therapeutic value of recombinant AP3S2 protein is mainly concentrated in the fields of tumor and inflammation.
AP3S2 is involved in intracellular protein transport, which can directly affect the growth and proliferation of cancer cells. It can also promote the immune system to clear cancer cells.
The combination of AP3S2 and other therapeutic agents can have a better therapeutic effect, but the specific effect needs to be further studied.
The optimal storage condition of AP3S2 is the refrigerator at -20℃, avoid light and moisturize, and do not repeatedly freeze and thaw.
Recombinant AP3S2 protein is involved in intracellular immune regulation and can affect the tubule system, cytophagocytosis and the formation of acidic lysosomes.
The AP3S2 is involved in protein transport from the Golgi apparatus to the endoplasmic reticulum during intracellular transport.
Recombinant AP3S2 protein in mouse model can significantly promote tumor growth inhibition and reduce tumor metastasis.
The cost of synthesis of AP3S2 varies according to different manufacturers, methods and other factors.
At present, the preclinical research of AP3S2 mainly focuses on in vivo and in vitro experiments and animal model studies.
AP3S2 can be prepared by synthesis, expression and purification.
The preparation difficulty of AP3S2 depends on the specific preparation method and experimental conditions.
Customer Reviews (4)
Write a reviewGreen production process of AP3S2 is very advanced, using the most environmentally friendly production process and is very friendly to the environment.
They have a wide range of products and can always find the AP3S2 we need.
Catalytic activity of AP3S2 is really amazing, and it can bring unexpected catalytic effects every time it is used, and its performance is impressive.
The reproducibility of AP3S2 can be said to be almost 100 percent, and the results are very consistent in every experiment or application, which is an important indication of its excellent quality.
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