Recombinant Human AOX1 293 Cell Lysate

• Cat.No.: AOX1-8821HCL

Specification

• Description: Antigen standard for aldehyde oxidase 1 (AOX1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AOX1 aldehyde oxidase 1 [ Homo sapiens ]
• Official Symbol: AOX1
• Synonyms: AOX1; aldehyde oxidase 1; aldehyde oxidase; AO; AOH1
• Gene ID: 316
• mRNA Refseq: NM_001159
• Protein Refseq: NP_001150
• MIM: 602841
• UniProt ID: Q06278
• Chromosome Location: 2q33
• Pathway: Drug metabolism - cytochrome P450, organism-specific biosystem; Drug metabolism - cytochrome P450, conserved biosystem; Metabolic pathways, organism-specific biosystem; Nicotinate and nicotinamide metabolism, organism-specific biosystem; Nicotinate and nicotinamide metabolism, conserved biosystem; Nicotine metabolism, organism-specific biosystem; Tryptophan metabolism, organism-specific biosystem
• Function: 2 iron, 2 sulfur cluster binding; NAD binding; UDP-N-acetylmuramate dehydrogenase activity; aldehyde oxidase activity; electron carrier activity; flavin adenine dinucleotide binding; iron ion binding; metal ion binding; molybdopterin cofactor binding; oxidoreductase activity, acting on CH-OH group of donors; xanthine dehydrogenase activity

Reviews

05/16/2020

Using AOX1 can reduce the error in the experiment.

05/02/2020

Preserving its stability under extreme conditions, such as at high or low temperatures, is very advantageous for us to conduct experiments.

02/28/2020

Catalytic activity is very strong, which can significantly improve the selectivity and yield of chemical reactions, and has high application value.

09/27/2019

After using this recombinant protein, the lower limit of Western blot detection is greatly reduced.

Q&As

What is the pharmacological action of AOX1 ?

AOX1 can play anti-inflammatory, antioxidant and immunomodulatory roles in the body, and can produce therapeutic effects on many diseases.

Does AOX1 have an anti-inflammatory advantage?

AOX1 can reduce the body's inflammatory response, reduce the risk of infection, etc., is an effective anti-inflammatory drug.

What quality control is required for AOX1 ?

The quality control of AOX1 includes process control in the production process, evaluation of product quality characteristics and physical and chemical properties.

In what basic research is AOX1 applied?

AOX1 is used in basic research, such as cardiovascular diseases, immune system diseases, diabetes and other related research.

How is the clinical trial of AOX1 progressing?

AOX1 has been carried out a number of clinical trials, in the treatment of different diseases have achieved good efficacy.

What is the mechanism of action of AOX1 in cardiovascular diseases?

Recombinant AOX1 protein can coordinate the metabolism of oxygen free radicals and trans-lipid peroxides in the body, thereby reducing the risk of cardiovascular disease.

Does the fermentation process of AOX1 need to be optimized?

The AOX1 fermentation process needs to be optimized for mass transfer, metabolism and expression to improve yield and quality.

What are the advantages of AOX1 in the field of new drug development?

AOX1 has the advantages of high safety and stable effect in the research and development of new drugs.

What are the structural characteristics of AOX1 ?

The AOX1 has a molecular weight of about 38kDa and is characterized by a cofactor FAD, which shows monoglycine dehydrogenase activity in this linked molecule.

What are the storage conditions of AOX1 ?

AOX1 can be stably preserved at 4 degrees of refrigeration, and long-term storage needs to be stored at an ultra-low temperature environment of -80 degrees.

Is there any relationship between AOX1 and lupus erythematosus?

AOX1 is an immunomodulatory protein that can produce therapeutic effects on autoimmune diseases such as lupus erythematosus.

What are the indications for AOX1 ?

AOX1 can be used in the treatment of cardiovascular diseases, lupus erythematosus, autoimmune diseases and other diseases.

Is the therapeutic effect of AOX1 durable?

The therapeutic effect of AOX1 has a certain persistence, and the treatment regimen can be adjusted according to the patient's condition.

Which clinical application prospect of AOX1 ?

As a biomedical product, AOX1 has broad clinical application prospects in cardiovascular diseases, immune system diseases, metabolic diseases and other fields.

What is the dosage and use method of AOX1 ?

The dosage and use method of AOX1 need to be guided by the doctor according to the specific disease and patient situation.

What is the action mechanism of AOX1 in the treatment of diabetes?

AOX1 can help reduce blood sugar, improve insulin resistance, etc., so as to have a therapeutic effect on diabetes.

Does the production process of AOX1 ?

The production process of AOX1 includes gene cloning, fermentation, purification and other steps. Generally, E. coli expression system and mammalian cell expression system are used.

What is the significance of AOX1 spectral analysis?

AOX1 spectral analysis can help researchers study protein structure and properties, explore its mechanism of action in biological and medical research, and provide an important reference for subsequent research.

How safe is AOX1 ?

As a biomedical product, AOX1 needs to be strictly evaluated for safety in clinical application to ensure its safety.

How to purify AOX1 ?

AOX1 is often purified by affinity chromatography, ion exchange chromatography, gel filtration and other methods.