"ANXA11" Related Products

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Recombinant Human ANXA11 293 Cell Lysate

Cat.No.: ANXA11-8836HCL
Description: Antigen standard for annexin A11 (ANXA11), transcript variant c is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ANXA11 annexin A11 [ Homo sapiens ]
Official Symbol: ANXA11
Synonyms: ANXA11; annexin A11; ANX11; CAP-50; annexin XI; annexin-11; 56 kDa autoantigen; autoantigen, 56-kD; calcyclin-associated annexin 50; CAP50;
Gene ID: 311
mRNA Refseq: NM_145869
Protein Refseq: NP_665876
MIM: 602572
UniProt ID: P50995
Chromosome Location: 10q22.3
Function: S100 alpha binding; calcium ion binding; calcium-dependent phospholipid binding; calcium-dependent protein binding; phosphatidylethanolamine binding; protein binding;

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