Recombinant Human AMIGO1 293 Cell Lysate
Cat.No. : | AMIGO1-8882HCL |
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- Gene Information
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Description : | Antigen standard for adhesion molecule with Ig-like domain 1 (AMIGO1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AMIGO1 adhesion molecule with Ig-like domain 1 [ Homo sapiens ] |
Official Symbol : | AMIGO1 |
Synonyms : | AMIGO; AMIGO-1 |
Gene ID : | 57463 |
mRNA Refseq : | NM_020703.2 |
Protein Refseq : | NP_065754.2 |
UniProt ID : | Q86WK6 |
Chromosome Location : | 1p13.3 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionAMIGO1 plays a critical role in promoting neurite outgrowth and axon guidance by regulating cytoskeletal dynamics and signaling pathways involved in axonal growth cone guidance.
AMIGO1 interacts with various binding partners, including cell adhesion molecules and ion channels, through both homophilic and heterophilic interactions, contributing to neuronal cell-cell communication and synaptic connectivity.
During neuronal development, AMIGO1 undergoes dynamic changes in its subcellular localization, transitioning from a primarily intracellular distribution to a more plasma membrane-associated localization.
AMIGO1 has been implicated in the regulation of neuronal migration during brain development, particularly in the formation of cortical layers and the establishment of neuronal circuitry.
AMIGO1 exerts a neuroprotective effect by promoting neuronal survival and inhibiting apoptosis through the activation of intracellular signaling pathways that regulate cell survival and anti-apoptotic factors.
AMIGO1 is involved in myelination processes and oligodendrocyte development, promoting the differentiation and maturation of oligodendrocytes and facilitating proper myelin sheath formation.
AMIGO1 shows a distinct tissue-specific expression pattern, with high expression in the brain, particularly in the developing and mature neurons, as well as in other organs such as the heart, kidney, and lung.
AMIGO1 has been shown to modulate synaptic plasticity by regulating the surface expression of neurotransmitter receptors and modulating synaptic strength and connectivity.
Altered expression or dysregulation of AMIGO1 has been associated with various neurological disorders, including epilepsy, schizophrenia, and autism spectrum disorders, suggesting its potential involvement in disease pathogenesis.
Modulation of AMIGO1 expression or activity represents a potential therapeutic target for neurological conditions, and further studies investigating the specific mechanisms and downstream signaling pathways regulated by AMIGO1 may unveil novel therapeutic strategies for these disorders.
Customer Reviews (3)
Write a reviewSensitivity in detecting low-abundance proteins.
Specific target detection in various tissue types.
Robust assay performance in high-throughput screening.
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