Recombinant Human AMDHD1 293 Cell Lysate

• Cat.No.: AMDHD1-8885HCL

Specification

• Description: Antigen standard for amidohydrolase domain containing 1 (AMDHD1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AMDHD1 amidohydrolase domain containing 1 [ Homo sapiens ]
• Official Symbol: AMDHD1
• Synonyms: AMDHD1; amidohydrolase domain containing 1; probable imidazolonepropionase; MGC35366; amidohydrolase domain-containing protein 1; HMFT1272
• Gene ID: 144193
• mRNA Refseq: NM_152435
• Protein Refseq: NP_689648
• UniProt ID: Q96NU7
• Chromosome Location: 12q23.1
• Pathway: Histidine catabolism, organism-specific biosystem; Histidine metabolism, organism-specific biosystem; Histidine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; histidine degradation III, organism-specific biosystem
• Function: imidazolonepropionase activity; metal ion binding

Reviews

11/23/2022

Compatibility with challenging sample types (e.g., crude extracts).

04/08/2020

Accurate determination of protein complex composition.

05/03/2019

Reliable detection of protein-protein interaction dynamics.