"AMBRA1" Related Products

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Recombinant Human AMBRA1 293 Cell Lysate

Cat.No.: AMBRA1-8887HCL
Description: Antigen standard for autophagy/beclin-1 regulator 1 (AMBRA1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: AMBRA1 autophagy/beclin-1 regulator 1 [ Homo sapiens ]
Official Symbol: AMBRA1
Synonyms: AMBRA1; autophagy/beclin-1 regulator 1; activating molecule in BECN1-regulated autophagy protein 1; DCAF3; DDB1 and CUL4 associated factor 3; FLJ20294; KIAA1736; WD repeat domain 94; WDR94; activating molecule in beclin-1-regulated autophagy; MGC33725;
Gene ID: 55626
mRNA Refseq: NM_017749
Protein Refseq: NP_060219
MIM: 611359
UniProt ID: Q9C0C7
Chromosome Location: 11p11.2
Pathway: Senescence and Autophagy, organism-specific biosystem;

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