Recombinant Human ALKBH7 293 Cell Lysate

• Cat.No.: ALKBH7-8899HCL

Specification

• Description: Antigen standard for alkB, alkylation repair homolog 7 (E. coli) (ALKBH7) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ALKBH7 alkB, alkylation repair homolog 7 (E. coli) [ Homo sapiens ]
• Official Symbol: ALKBH7
• Synonyms: ABH7; SPATA11; UNQ6002
• Gene ID: 84266
• mRNA Refseq: NM_032306.3
• Protein Refseq: NP_115682.1
• MIM: 613305
• UniProt ID: Q9BT30
• Chromosome Location: 19p13.3
• Function: metal ion binding;oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors;oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen

Reviews

08/20/2021

Growth factor activity that promotes cell growth and proliferation.

07/14/2021

Easy to use, high stability, significant effect.

01/29/2020

It has good application performance in immunodetection, such as ELISA, Western blot, etc.

04/19/2019

Synthesized with high efficiency and yield.

Q&As

Is ALKBH7 related to the nervous system?

Yes, the ALKBH7 gene has been shown to be important for the proper maintenance of nervous system development and function.

What is the expression pattern of ALKBH7 gene?

The expression pattern of ALKBH7 gene showed broad, tissue specific and period specific.

What is the relationship between ALKBH7 gene and liver cancer?

Some studies have found that ALKBH7 gene is highly expressed in liver cancer cells and is closely related to the progression and prognosis of liver cancer.

Does mutation of ALKBH7 gene cause disease?

Yes, some studies suggest that mutations in the ALKBH7 gene may promote the development and spread of tumors and may contribute to the development of related diseases such as neurological disorders.

What is the function of the protein product of ALKBH7 gene?

The mRNA cap reader protein recognizes and binds to the 3' end of a methylated RNA molecule. Its function is to help transcription factors effectively bind to mRNA, thus promoting the normal process of protein production.

What diseases is the ALKBH7 gene associated with?

Some diseases that are known to be related to the ALKBH7 gene include liver cancer, pancreatic cancer, lung cancer, schizophrenia, and Alzheimer's disease.

What are the inhibitors of ALKBH7 gene?

At present, some inhibitors targeting ALKBH7 gene have been developed, such as plant silicates and so on.

How cellular processes does the ALKBH7 gene regulate?

ALKBH7 regulates RNA methylation and demethylation, and is involved in cellular processes such as RNA stability, transcription and translation.

In which tissues is ALKBH7 gene highly expressed?

ALKBH7 gene is highly expressed in sperm, but the expression rate of ALKBH7 gene in other tissues and cell types varies with physiological activity and different developmental stages.

Which signaling pathways does the ALKBH7 gene regulate?

ALKBH7 gene has been shown to participate in ATM/ATR signaling pathway, Wnt signaling pathway, NF-κB signaling pathway, etc.

Is the genetic mutation of ALKBH7 gene heritable?

Yes, mutations in the ALKBH7 gene are heritable.

Is ALKBH7 involved in DNA repair?

Although the ALKBH7 gene is widely expressed in multiple tissues, it has not been found to be involved in DNA repair processes, focusing on its role in RNA stability and transcription.

Is ALKBH7 involved in cancer?

Studies of the ALKBH7 gene suggest that it may be involved in some cancers and tumours, but more extensive research is needed.