"ALKBH5" Related Products

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Recombinant Human ALKBH5 293 Cell Lysate

Cat.No.: ALKBH5-8900HCL
Description: Antigen standard for alkB, alkylation repair homolog 5 (E. coli) (ALKBH5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ALKBH5 alkB, alkylation repair homolog 5 (E. coli) [ Homo sapiens ]
Official Symbol: ALKBH5
Synonyms: ALKBH5; alkB, alkylation repair homolog 5 (E. coli); OFOXD1, oxoglutarate and iron dependent oxygenase domain containing; probable alpha-ketoglutarate-dependent dioxygenase ABH5; FLJ20308; alkylated DNA repair protein alkB homolog 5; oxoglutarate and iron-dependent oxygenase domain containing; ABH5; OFOXD; OFOXD1;
Gene ID: 54890
mRNA Refseq: NM_017758
Protein Refseq: NP_060228
MIM: 613303
UniProt ID: Q6P6C2
Chromosome Location: 17p11.2
Function: metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen;

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