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Recombinant Human ALG8 293 Cell Lysate

Cat.No. : ALG8-8905HCL
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  • Gene Information
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Description : Antigen standard for asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ALG8 asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol : ALG8
Synonyms : ALG8; asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae); asparagine linked glycosylation 8 homolog (yeast, alpha 1,3 glucosyltransferase); probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase; MGC2840; HUSSY-02; asparagine-linked glycosylation protein 8 homolog; dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase; dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase; dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase; dolichyl-P-glucose:Glc1Man9GlcNAc2-PP-dolichyl-alpha-1,3-glucosyltransferase; asparagine-linked glycosylation 8 homolog (yeast, alpha-1,3-glucosyltransferase); asparagine-linked glycosylation 8 homolog (S. cerevisiae, alpha-1,3-glucosyltransferase); CDG1H;
Gene ID : 79053
mRNA Refseq : NM_001007027
Protein Refseq : NP_001007028
MIM : 608103
UniProt ID : Q9BVK2
Chromosome Location : 11pter-p15.5
Pathway : Asparagine N-linked glycosylation, organism-specific biosystem; Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; N-Glycan biosynthesis, organism-specific biosystem; N-Glycan biosynthesis, conserved biosystem; N-glycan precursor biosynthesis, organism-specific biosystem;
Function : alpha-1,3-mannosyltransferase activity; transferase activity, transferring hexosyl groups;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (17)

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How does Alg8 protein participate in protein glycosylation? 03/27/2023

Alg8 protein is involved in n-biglycosylation and leads the control of n-glycosylation of single amido group, and is the key enzyme of glycosylation on the polypeptide chain.

In which cell structures is Alg8 protein expressed? 12/27/2022

The protein is mainly expressed in endoplasmic reticulum and plays an important role in glycosylation reaction on endoplasmic reticulum.

In what physiological processes does Alg8 protein play a role? 10/27/2022

Alg8 protein plays an important role in a variety of biological and medical research, such as calcium ion transport, nervous system development and other fields.

What is the relationship between Alg8 protein and cell signal transduction? 09/18/2022

Alg8 protein is involved in cell signal transduction because its glycosylation activity is involved in the regulation and control of many signaling pathways.

What diseases is Alg8 associated with? 11/29/2021

Alg8 protein plays an important role in many diseases, such as congenital heart disease, Crohn's disease, autoimmune diseases, vascular endothelial dysfunction and so on.

How is Alg8 transported to the cell membrane? 10/11/2021

Through the endoplasmic reticulum, Alg8 protein enters the Golgi apparatus and its transport and distribution are detected by synchronous multi-infiltration method and electron microscopy. Vesicles and membranes that can enter the Golgi apparatus gradually form aggregation, and gradually separate and pass through the Golgi apparatus membrane with increasing density, and play a role in the cell membrane.

What is the evolution of Alg8 protein? 09/02/2021

Alg8 protein may have appeared in plastid in ancient times, changed gradually with the evolution of organisms, and played an increasingly important role in the process of evolution.

What is the expression pattern of Alg8 protein? 03/05/2021

Alg8 protein is widely expressed in many tissues and organs of the human body, and some studies have shown that there is a higher level of expression in liver, kidney and other organs.

Does Alg8 protein participate in the regulation of immune function? 03/04/2021

Alg8 protein is one of many cell surface glycosylase, so it may be involved in the regulation and regulation of immune function.

What are the structural characteristics of Alg8 protein? 11/28/2020

Alg8 proteins belong to a family of transmembrane proteins that structurally contain an n-terminal signaling peptide, a transmembrane domain, a C-terminal extracellular group, and a set of highly conserved enzyme active sites.

What diseases can the loss of Alg8 protein cause? 06/15/2020

Loss of Alg8 protein may cause birth defects, mental retardation, ataxia, and neurological disorders.

In what signaling pathways does the Alg8 protein play a role? 04/10/2020

This protein plays a role in a variety of signaling pathways, including NF-κB signal, Jak/Stat signal, PI3K/Akt signal, etc., which may have a certain impact on the normal regulation and function of these pathways.

What is the genomic structure of Alg8 protein? 10/09/2019

The Alg8 gene is located on chromosome 17p11.2 and contains 7 exons and 6 introns with a total length of 23 kb.

How do changes in Alg8 protein expression affect cell function? 08/27/2019

Changes in the expression level of Alg8 protein may affect the degree of cell glycosylation, and thus affect normal cell function and physiological processes.

What are the semantic homologues of Alg8 protein? 07/25/2019

The semantic ortholog of Alg8 protein is Drosophila melanogaster CG32815.

What is the application prospect of Alg8 protein in cancer treatment? 07/13/2019

Alg8 protein has certain potential in anti-cancer therapy because it plays an important role in the process of cellular glycosylation, and studying its regulation in tumor cells may help to study the mechanism of cancer occurrence.

What factors regulate Alg8 protein? 06/29/2019

Alg8 protein is regulated by many factors, including transcription factors, RNA splicing and transformation, which affect the expression level and activity of Alg8.

Customer Reviews (4)

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Reviews
09/20/2022

    alg8 has good stability in solution and is not easily aggregated or inactivated.

    03/04/2020

      The activity of alg8 was similar to that of the native protein and was able to perform the expected biological functions.

      08/13/2019

        High expression efficiency and can efficiently synthesize a large number of target proteins in host cells.

        08/08/2019

          alg8 was highly purified and not doped with other proteins or impurities.

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