"AICDA" Related Products

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Recombinant Human AICDA 293 Cell Lysate

Cat.No.: AICDA-8958HCL
Description: Antigen standard for activation-induced cytidine deaminase (AICDA) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: AICDA activation-induced cytidine deaminase [ Homo sapiens ]
Official Symbol: AICDA
Synonyms: AICDA; activation-induced cytidine deaminase; AID; ARP2; CDA2; HIGM2; cytidine aminohydrolase; integrated into Burkitts lymphoma cell line Ramos;
Gene ID: 57379
mRNA Refseq: NM_020661
Protein Refseq: NP_065712
MIM: 605257
UniProt ID: Q9GZX7
Chromosome Location: 12p13
Pathway: IL4-mediated signaling events, organism-specific biosystem; Intestinal immune network for IgA production, organism-specific biosystem; Intestinal immune network for IgA production, conserved biosystem; Primary immunodeficiency, organism-specific biosystem; Primary immunodeficiency, conserved biosystem; salvage pathways of pyrimidine deoxyribonucleotides, organism-specific biosystem; salvage pathways of pyrimidine ribonucleotides, organism-specific biosystem;
Function: cytidine deaminase activity; cytidine deaminase activity; hydrolase activity; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines; metal ion binding; protein binding; zinc ion binding;

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