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Recombinant Human AHSP 293 Cell Lysate

Cat.No. : AHSP-8959HCL
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  • Gene Information
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Description : Antigen standard for alpha hemoglobin stabilizing protein (AHSP) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AHSP alpha hemoglobin stabilizing protein [ Homo sapiens ]
Official Symbol : AHSP
Synonyms : AHSP; alpha hemoglobin stabilizing protein; ERAF, erythroid associated factor; alpha-hemoglobin-stabilizing protein; alpha hemoglobin stabilising protein; EDRF; erythroid associated factor; erythroid-associated factor; erythroid differentiation-related factor; erythroid differentiation associated factor; ERAF;
Gene ID : 51327
mRNA Refseq : NM_016633
Protein Refseq : NP_057717
MIM : 605821
UniProt ID : Q9NZD4
Chromosome Location : 16p11.1
Function : hemoglobin binding; unfolded protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What techniques are used to quantify the binding affinity between Ahsp and alpha-globin? 04/25/2022

echniques such as isothermal titration calorimetry (ITC) and surface plasmon resonance (SPR) are commonly employed to quantify the binding affinity and kinetics of Ahsp-alpha-globin interactions.

What is the connection between Ahsp and hemoglobinopathies? 01/20/2022

Ahsp deficiency can lead to unstable alpha-globin chains, contributing to the development of certain hemoglobinopathies like alpha-thalassemia.

How does Ahsp respond to cellular stress conditions? 11/17/2021

Ahsp expression may be upregulated during cellular stress to enhance its protective role in stabilizing alpha-globin chains and preventing protein aggregation.

What techniques are commonly used to study the interactions of Ahsp with alpha-globin chains? 03/09/2021

Techniques such as nuclear magnetic resonance (NMR), X-ray crystallography, and molecular dynamics simulations are commonly used to study protein-protein interactions involving Ahsp and alpha-globin.

How does Ahsp interact with chaperone proteins in the cell? 10/31/2020

Ahsp can interact with molecular chaperones like Hsp70 to assist in protein folding and prevent misfolding of alpha-globin chains.

How does Ahsp achieve its stabilizing function at the molecular level? 06/15/2020

Ahsp binds to free alpha-globin chains, preventing their aggregation and degradation by maintaining their folded state.

What is the significance of Ahsp in preventing thalassemia? 09/28/2019

Ahsp plays a role in preventing excess alpha-globin chains from aggregating and precipitating, which is a key factor in the pathogenesis of alpha-thalassemia.

How does Ahsp's function compare to other molecular chaperones in the cell? 07/12/2019

Ahsp's role is unique, focusing specifically on stabilizing alpha-globin chains, whereas other chaperones have broader functions in assisting protein folding and transport.

Can you explain the role of Ahsp in the context of iron metabolism? 12/04/2018

Ahsp indirectly influences iron metabolism by stabilizing alpha-globin chains and preventing their degradation, which in turn maintains balanced globin chain production and minimizes iron waste.

How does Ahsp contribute to maintaining red blood cell stability and lifespan? 09/03/2018

Ahsp's role in stabilizing alpha-globin chains helps prevent the formation of toxic aggregates that could damage red blood cells and shorten their lifespan.

Customer Reviews (5)

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Reviews
05/13/2022

    Outstanding product supplement. Worth every penny and arrived ahead of schedule.

    03/02/2021

      Top-notch lab protein for a fraction of the cost. Superb value for money.

      07/06/2020

        Great protein quality at an affordable price. Impressed with the results!

        12/27/2019

          Exceptional quality product that doesn't break the bank. Fast delivery too!

          09/18/2018

            This protein product is a game-changer – premium quality and budget-friendly.

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