Recombinant Human AHSP 293 Cell Lysate
Cat.No. : | AHSP-8959HCL |
- Specification
- Gene Information
- Related Products
Description : | Antigen standard for alpha hemoglobin stabilizing protein (AHSP) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AHSP alpha hemoglobin stabilizing protein [ Homo sapiens ] |
Official Symbol : | AHSP |
Synonyms : | AHSP; alpha hemoglobin stabilizing protein; ERAF, erythroid associated factor; alpha-hemoglobin-stabilizing protein; alpha hemoglobin stabilising protein; EDRF; erythroid associated factor; erythroid-associated factor; erythroid differentiation-related factor; erythroid differentiation associated factor; ERAF; |
Gene ID : | 51327 |
mRNA Refseq : | NM_016633 |
Protein Refseq : | NP_057717 |
MIM : | 605821 |
UniProt ID : | Q9NZD4 |
Chromosome Location : | 16p11.1 |
Function : | hemoglobin binding; unfolded protein binding; |
Products Types
◆ Recombinant Protein | ||
AHSP-0103H | Recombinant Human AHSP Protein (Met1-Ser102), His-tagged | +Inquiry |
Ahsp-616R | Recombinant Rat Ahsp Protein, His/GST-tagged | +Inquiry |
AHSP-614H | Recombinant Human AHSP Protein, His-tagged | +Inquiry |
aHSP-3367H | Recombinant Human aHSP, His-tagged, S tagged | +Inquiry |
Ahsp-615M | Recombinant Mouse Ahsp Protein, His-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionechniques such as isothermal titration calorimetry (ITC) and surface plasmon resonance (SPR) are commonly employed to quantify the binding affinity and kinetics of Ahsp-alpha-globin interactions.
Ahsp deficiency can lead to unstable alpha-globin chains, contributing to the development of certain hemoglobinopathies like alpha-thalassemia.
Ahsp expression may be upregulated during cellular stress to enhance its protective role in stabilizing alpha-globin chains and preventing protein aggregation.
Techniques such as nuclear magnetic resonance (NMR), X-ray crystallography, and molecular dynamics simulations are commonly used to study protein-protein interactions involving Ahsp and alpha-globin.
Ahsp can interact with molecular chaperones like Hsp70 to assist in protein folding and prevent misfolding of alpha-globin chains.
Ahsp binds to free alpha-globin chains, preventing their aggregation and degradation by maintaining their folded state.
Ahsp plays a role in preventing excess alpha-globin chains from aggregating and precipitating, which is a key factor in the pathogenesis of alpha-thalassemia.
Ahsp's role is unique, focusing specifically on stabilizing alpha-globin chains, whereas other chaperones have broader functions in assisting protein folding and transport.
Ahsp indirectly influences iron metabolism by stabilizing alpha-globin chains and preventing their degradation, which in turn maintains balanced globin chain production and minimizes iron waste.
Ahsp's role in stabilizing alpha-globin chains helps prevent the formation of toxic aggregates that could damage red blood cells and shorten their lifespan.
Customer Reviews (5)
Write a reviewOutstanding product supplement. Worth every penny and arrived ahead of schedule.
Top-notch lab protein for a fraction of the cost. Superb value for money.
Great protein quality at an affordable price. Impressed with the results!
Exceptional quality product that doesn't break the bank. Fast delivery too!
This protein product is a game-changer – premium quality and budget-friendly.
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