Recombinant Human AFTPH 293 Cell Lysate
Cat.No. : | AFTPH-8985HCL |
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- Gene Information
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Description : | Antigen standard for aftiphilin (AFTPH), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AFTPH aftiphilin [ Homo sapiens ] |
Official Symbol : | AFTPH |
Synonyms : | Nbla10388 |
Gene ID : | 54812 |
mRNA Refseq : | NM_001002243 |
Protein Refseq : | NP_001002243 |
UniProt ID : | Q6ULP2 |
Chromosome Location : | 2p14 |
Function : | clathrin binding; |
Products Types
◆ Recombinant Protein | ||
AFTPH-93R | Recombinant Rhesus Macaque AFTPH Protein, His (Fc)-Avi-tagged | +Inquiry |
AFTPH-265R | Recombinant Rhesus monkey AFTPH Protein, His-tagged | +Inquiry |
◆ Lysates | ||
AFTPH-8986HCL | Recombinant Human AFTPH 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionThere is currently no known association between disease and mutations in the AFTPH gene.
AFTPH protein participates in the remodeling of the cytoskeleton and dynamic regulation of the cell membrane, affecting cellular morphology.
AFTPH protein interacts with multiple signaling proteins, cytoskeletal proteins, and transcription factors.
AFTPH protein interacts with various signaling pathways and protein complexes, influencing cellular signal transduction and dynamics.
AFTPH protein regulates cellular functions by activating signaling pathways like PI3K/Akt and MAPK.
AFTPH protein is mainly localized in the cytoplasm and participates in trafficking processes involving the endoplasmic reticulum and Golgi apparatus.
The expression of AFTPH protein is regulated through transcriptional and post-transcriptional mechanisms.
AFTPH protein is involved in regulating various cellular processes, such as migration, adhesion, proliferation, and signaling.
The precise physiological roles of AFTPH protein in embryonic development, tissue homeostasis, and tumor progression require further investigation.
Inhibiting the expression or activity of AFTPH protein can modulate processes like cell migration, proliferation, and tumor development.
Customer Reviews (3)
Write a reviewMapping protein-protein interactions in extracellular matrix assembly for tissue integrity.
Studying protein-protein interactions in receptor signaling for cellular communication.
Probing protein-protein interactions in ion channel function for neuronal excitability.
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