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Recombinant Human AFTPH 293 Cell Lysate

Cat.No. : AFTPH-8985HCL
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  • Gene Information
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Description : Antigen standard for aftiphilin (AFTPH), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AFTPH aftiphilin [ Homo sapiens ]
Official Symbol : AFTPH
Synonyms : Nbla10388
Gene ID : 54812
mRNA Refseq : NM_001002243
Protein Refseq : NP_001002243
UniProt ID : Q6ULP2
Chromosome Location : 2p14
Function : clathrin binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Are there disease-associated mutations in the AFTPH gene? 04/20/2022

There is currently no known association between disease and mutations in the AFTPH gene.

How does AFTPH protein influence cellular morphology and cytoskeleton? 07/24/2021

AFTPH protein participates in the remodeling of the cytoskeleton and dynamic regulation of the cell membrane, affecting cellular morphology.

Which molecules interact with AFTPH protein? 02/07/2021

AFTPH protein interacts with multiple signaling proteins, cytoskeletal proteins, and transcription factors.

How does AFTPH protein contribute to cellular signal transduction networks and protein complexes? 04/11/2020

AFTPH protein interacts with various signaling pathways and protein complexes, influencing cellular signal transduction and dynamics.

How does AFTPH protein regulate cellular functions through signaling pathways? 06/17/2018

AFTPH protein regulates cellular functions by activating signaling pathways like PI3K/Akt and MAPK.

What is the subcellular localization and trafficking mechanism of AFTPH protein? 10/22/2017

AFTPH protein is mainly localized in the cytoplasm and participates in trafficking processes involving the endoplasmic reticulum and Golgi apparatus.

How is the expression of AFTPH protein regulated? 08/16/2017

The expression of AFTPH protein is regulated through transcriptional and post-transcriptional mechanisms.

What is the functional role of AFTPH protein? 06/04/2017

AFTPH protein is involved in regulating various cellular processes, such as migration, adhesion, proliferation, and signaling.

What are the physiological roles of AFTPH protein in development, tissue homeostasis, or disease progression? 04/09/2016

The precise physiological roles of AFTPH protein in embryonic development, tissue homeostasis, and tumor progression require further investigation.

Can manipulating AFTPH protein modulate specific cellular functions or signaling pathways? 08/11/2015

Inhibiting the expression or activity of AFTPH protein can modulate processes like cell migration, proliferation, and tumor development.

Customer Reviews (3)

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08/30/2022

    Mapping protein-protein interactions in extracellular matrix assembly for tissue integrity.

    06/09/2021

      Studying protein-protein interactions in receptor signaling for cellular communication.

      03/23/2018

        Probing protein-protein interactions in ion channel function for neuronal excitability.

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