Recombinant Human AFAP1 293 Cell Lysate
Cat.No. : | AFAP1-8990HCL |
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- Gene Information
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Description : | Antigen standard for actin filament associated protein 1 (AFAP1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AFAP1 actin filament associated protein 1 [ Homo sapiens ] |
Official Symbol : | AFAP1 |
Synonyms : | AFAP1; actin filament associated protein 1; actin filament-associated protein 1; AFAP; AFAP 110; 110 kDa actin filament-associated protein; actin filament-associated protein, 110 kDa; AFAP110; AFAP-110; FLJ56849; |
Gene ID : | 60312 |
mRNA Refseq : | NM_198595 |
Protein Refseq : | NP_940997 |
MIM : | 608252 |
UniProt ID : | Q8N556 |
Chromosome Location : | 4p16 |
Function : | actin binding; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionAFAP1 protein contributes to disease development or pathophysiological conditions, and the underlying molecular mechanisms are currently being investigated.
Dysregulation or dysfunction of AFAP1 protein can disrupt cellular homeostasis, perturb molecular signaling networks, and contribute to disease progression.
AFAP1 protein holds potential as a target for modulating cellular processes, and further research is needed to explore its therapeutic implications and potential interventions.
AFAP1 protein interacts with specific molecules or forms protein complexes, which have functional implications in various cellular processes.
AFAP1 protein plays a role in cellular processes such as cytoskeletal dynamics, cell migration, or signal transduction, and its function can be investigated experimentally using techniques like siRNA knockdown or overexpression studies.
The subcellular localization of AFAP1 protein can be experimentally determined using techniques such as immunofluorescence microscopy or subcellular fractionation.
Various experimental techniques or assays, such as co-immunoprecipitation, live-cell imaging, or functional assays, have been used to study the functional significance of AFAP1 protein.
Post-translational modifications and regulatory mechanisms modulate the activity or stability of AFAP1 protein.
Genetic variations or mutations in the gene encoding AFAP1 protein may impact its expression or function, influencing cellular processes or disease susceptibility.
The expression of AFAP1 protein is regulated, and specific factors or signaling pathways are involved in its expression control.
Customer Reviews (3)
Write a reviewUnraveling protein-protein interactions in RNA processing for gene expression control.
Mapping protein-protein interactions in DNA transcription for gene regulation.
Investigating protein-protein interactions in autophagy pathways for cellular homeostasis.
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