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Recombinant Human ADD1 293 Cell Lysate

Cat.No. : ADD1-9017HCL
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  • Gene Information
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Description : Antigen standard for adducin 1 (alpha) (ADD1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ADD1 adducin 1 (alpha) [ Homo sapiens ]
Official Symbol : ADD1
Synonyms : ADD1; adducin 1 (alpha); alpha-adducin; erythrocyte adducin alpha subunit; ADDA; MGC3339; MGC44427;
Gene ID : 118
mRNA Refseq : NM_014189
Protein Refseq : NP_054908
MIM : 102680
UniProt ID : P35611
Chromosome Location : 4p16.3
Pathway : Activation of Chaperone Genes by XBP1(S), organism-specific biosystem; Activation of Chaperones by IRE1alpha, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Caspase-mediated cleavage of cytoskeletal proteins, organism-specific biosystem; Diabetes pathways, organism-specific biosystem;
Function : actin binding; actin filament binding; calmodulin binding; metal ion binding; protein heterodimerization activity; protein homodimerization activity; spectrin binding; transcription factor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Can we develop specific antibodies or probes to detect and visualize the localization of ADD1 in live cells or tissues? 10/27/2021

Specific antibodies or probes can be developed to visualize the localization of ADD1 in live cells or tissues.

What is the impact of altering the expression level or activity of ADD1 on cellular processes such as cell proliferation, differentiation, or apoptosis? 04/29/2021

Altering the expression level or activity of ADD1 can affect cellular processes like cell proliferation, differentiation, or apoptosis.

How does the expression of ADD1 change under various physiological or pathological conditions? 04/04/2021

The expression of ADD1 can change under different physiological or pathological conditions.

What is the subcellular localization of ADD1 within the cell? Is it predominantly found in the nucleus, cytoplasm, or other cellular compartments? 01/12/2021

ADD1 exhibits subcellular localization in various cellular compartments, such as the nucleus or cytoplasm.

What are the interacting partners or binding proteins of ADD1 in a specific cellular context? 10/15/2020

ADD1 interacts with specific binding proteins or partners in a context-dependent manner.

What is the tissue-specific expression pattern of ADD1 in different organs or cell types? 12/06/2018

The tissue-specific expression pattern of ADD1 varies across organs and cell types.

Are there any post-translational modifications (e.g., phosphorylation, acetylation) that regulate the activity or stability of ADD1? 06/14/2018

ADD1 undergoes post-translational modifications, including phosphorylation and acetylation, which can impact its activity and stability.

Are there any small molecule inhibitors or activators that can modulate the function of ADD1? What is their mechanism of action? 08/19/2017

There may exist small molecule inhibitors or activators that can modulate ADD1's function, with distinct mechanisms of action.

Can we identify the specific domains or motifs within ADD1 that are responsible for its functional activities? 07/04/2016

Specific domains or motifs within ADD1 are responsible for its functional activities.

What are the downstream target genes or signaling pathways regulated by ADD1, and how do they contribute to its biological functions? 01/21/2016

ADD1 regulates downstream target genes or signaling pathways that contribute to its biological functions.

Customer Reviews (3)

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Reviews
01/01/2019

    Highly effective in detecting and quantifying the expression levels of fusion proteins in protein expression analysis.

    06/09/2017

      Highly specific in detecting the target protein in tissue sections in immunohistochemistry experiments.

      11/01/2016

        Demonstrates high selectivity and sensitivity in detecting protein-protein interactions in pull-down assays.

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