Recombinant Human ADD1 293 Cell Lysate
Cat.No. : | ADD1-9017HCL |
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- Gene Information
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Description : | Antigen standard for adducin 1 (alpha) (ADD1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ADD1 adducin 1 (alpha) [ Homo sapiens ] |
Official Symbol : | ADD1 |
Synonyms : | ADD1; adducin 1 (alpha); alpha-adducin; erythrocyte adducin alpha subunit; ADDA; MGC3339; MGC44427; |
Gene ID : | 118 |
mRNA Refseq : | NM_014189 |
Protein Refseq : | NP_054908 |
MIM : | 102680 |
UniProt ID : | P35611 |
Chromosome Location : | 4p16.3 |
Pathway : | Activation of Chaperone Genes by XBP1(S), organism-specific biosystem; Activation of Chaperones by IRE1alpha, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Caspase-mediated cleavage of cytoskeletal proteins, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; |
Function : | actin binding; actin filament binding; calmodulin binding; metal ion binding; protein heterodimerization activity; protein homodimerization activity; spectrin binding; transcription factor binding; |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionSpecific antibodies or probes can be developed to visualize the localization of ADD1 in live cells or tissues.
Altering the expression level or activity of ADD1 can affect cellular processes like cell proliferation, differentiation, or apoptosis.
The expression of ADD1 can change under different physiological or pathological conditions.
ADD1 exhibits subcellular localization in various cellular compartments, such as the nucleus or cytoplasm.
ADD1 interacts with specific binding proteins or partners in a context-dependent manner.
The tissue-specific expression pattern of ADD1 varies across organs and cell types.
ADD1 undergoes post-translational modifications, including phosphorylation and acetylation, which can impact its activity and stability.
There may exist small molecule inhibitors or activators that can modulate ADD1's function, with distinct mechanisms of action.
Specific domains or motifs within ADD1 are responsible for its functional activities.
ADD1 regulates downstream target genes or signaling pathways that contribute to its biological functions.
Customer Reviews (3)
Write a reviewHighly effective in detecting and quantifying the expression levels of fusion proteins in protein expression analysis.
Highly specific in detecting the target protein in tissue sections in immunohistochemistry experiments.
Demonstrates high selectivity and sensitivity in detecting protein-protein interactions in pull-down assays.
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