"ASIC3" Related Products


Recombinant Human ACCN3 293 Cell Lysate

Cat.No.: ASIC3-9100HCL
Description: Antigen standard for amiloride-sensitive cation channel 3 (ACCN3), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ASIC3?acid-sensing (proton-gated) ion channel 3 [?Homo sapiens?(human) ]
Official Symbol: ASIC3
Synonyms: ASIC3; acid-sensing (proton-gated) ion channel 3; ACCN3; TNaC1; DRASIC; SLNAC1; acid-sensing ion channel 3; testis sodium channel 1; modulatory subunit of ASIC2a; proton-gated cation channel subunit; amiloride-sensitive cation channel 3, testis; neuronal amiloride-sensitive cation channel 3
Gene ID: 9311
mRNA Refseq: NM_004769
Protein Refseq: NP_004760
MIM: 611741
UniProt ID: Q9UHC3
Chromosome Location: 7q35
Pathway: Inflammatory mediator regulation of TRP channels, organism-specific biosystem; Ion channel transport, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem
Function: PDZ domain binding; cation channel activity; enterobactin transporter activity

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