Recombinant Human ACCN1 293 Cell Lysate


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Recombinant Human ACCN1 293 Cell Lysate

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Cat.No. : ASIC2-9103HCL
Description : Antigen standard for amiloride-sensitive cation channel 1, neuronal (ACCN1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ASIC2?acid-sensing (proton-gated) ion channel 2 [?Homo sapiens?(human) ]
Official Symbol : ASIC2
Synonyms : ASIC2; acid-sensing (proton-gated) ion channel 2; ACCN; BNC1; MDEG; ACCN1; BNaC1; ASIC2a; hBNaC1; acid-sensing ion channel 2; brain sodium channel 1; mammalian degenerin homolog; neuronal amiloride-sensitive cation channel 1; amiloride-sensitive cation channel 1, neuronal
Gene ID : 40
mRNA Refseq : NM_001094
Protein Refseq : NP_001085
MIM : 601784
UniProt ID : Q16515
Chromosome Location : 17q12
Pathway : Inflammatory mediator regulation of TRP channels, organism-specific biosystem; Ion channel transport, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem
Function : ion gated channel activity; ligand-gated sodium channel activity; protein binding; voltage-gated sodium channel activity
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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