Recombinant Human ACADSB 293 Cell Lysate

• Cat.No.: ACADSB-9112HCL

Specification

• Description: Antigen standard for acyl-Coenzyme A dehydrogenase, short/branched chain (ACADSB), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ACADSB acyl-CoA dehydrogenase, short/branched chain [ Homo sapiens ]
• Official Symbol: ACADSB
• Synonyms: ACADSB; acyl-CoA dehydrogenase, short/branched chain; ACAD7; SBCAD; 2-MEBCAD; short/branched chain specific acyl-CoA dehydrogenase, mitochondrial; 2-methylbutyryl-coenzyme A dehydrogenase; 2-methyl branched chain acyl-CoA dehydrogenase; 2 MEBCAD; 2 methyl branched chain acyl CoA dehydrogenase; 2 methylbutyryl CoA dehydrogenase; 2-methylbutyryl-CoA dehydrogenase; ACDSB_HUMAN; acyl CoA dehydrogenase, short/branched chain; OTTHUMP00000020685; OTTHUMP00000046795; Short/branched chain specific acyl-CoA dehydrogenase; acyl-Coenzyme A dehydrogenase, short/branched chain; 2-methylbutyryl-coenzyme A dehydrogenase; short/branched chain specific acyl-CoA dehydrogenase, mitochondrial; EC 1.3.99
• Gene ID: 36
• mRNA Refseq: NM_001609
• Protein Refseq: NP_001600
• MIM: 600301
• UniProt ID: P45954
• Chromosome Location: 10q26.13
• Pathway: Branched-chain amino acid catabolism, organism-specific biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Valine, leucine and isoleucine degradation, organism-specific biosystem; Valine, leucine and isoleucine degradation, conserved biosystem
• Function: acyl-CoA dehydrogenase activity; electron carrier activity; flavin adenine dinucleotide binding; short-branched-chain-acyl-CoA dehydrogenase activity

Reviews

05/07/2020

Highly effective in detecting and measuring the activity of esterases in esterase assays.

04/02/2018

Demonstrates excellent specificity and sensitivity in detecting the target protein in fluorescence-based assays.

Q&As

How does the activity of this protein contribute to cellular metabolism?

The activity of acyl-CoA dehydrogenase short/branched chain contributes to the breakdown of branched-chain amino acids and fatty acids to produce energy in cells.

Where is acyl-CoA dehydrogenase short/branched chain protein located within the cell?

Acyl-CoA dehydrogenase short/branched chain protein is located in the mitochondrial matrix.

What is the substrate specificity of this protein?

Acyl-CoA dehydrogenase short/branched chain protein has specificity for short/branched chain fatty acyl-CoA substrates.

What diseases or disorders are associated with mutations in this protein?

Mutations in this protein are associated with a variety of metabolic disorders, including isovaleric acidemia and 2-methylbutyryl-CoA dehydrogenase deficiency.

Are there any known inhibitors or activators of this protein?

There are currently no known inhibitors or activators of acyl-CoA dehydrogenase short/branched chain protein.

What are the physiological roles of acyl-CoA dehydrogenase short/branched chain?

Acyl-CoA dehydrogenase short/branched chain plays a key role in energy metabolism and the catabolism of branched-chain amino acids in cells.

What is the structure of this protein and how does it relate to its function?

Acyl-CoA dehydrogenase short/branched chain protein has a flavin adenine dinucleotide (FAD)-binding domain that is essential for its function.

How is the expression of acyl-CoA dehydrogenase short/branched chain regulated?

The expression of acyl-CoA dehydrogenase short/branched chain is regulated by a variety of factors, including gene expression and protein degradation pathways.

What research is currently being conducted to further understand the function of acyl-CoA dehydrogenase short/branched chain protein?

Research is ongoing to further understand the function and regulation of acyl-CoA dehydrogenase short/branched chain protein, as well as its role in metabolic disorders.

What is the function of acyl-CoA dehydrogenase short/branched chain protein?

Acyl-CoA dehydrogenase short/branched chain protein is involved in the metabolism of branched-chain amino acids and fatty acids.