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Recombinant Human AATF 293 Cell Lysate

Cat.No. : AATF-9155HCL
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  • Gene Information
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Description : Antigen standard for apoptosis antagonizing transcription factor (AATF) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AATF apoptosis antagonizing transcription factor [ Homo sapiens ]
Official Symbol : AATF
Synonyms : AATF; apoptosis antagonizing transcription factor; protein AATF; CHE 1; CHE1; DED; rb-binding protein Che-1; apoptosis-antagonizing transcription factor; CHE-1;
Gene ID : 26574
mRNA Refseq : NM_012138
Protein Refseq : NP_036270
MIM : 608463
UniProt ID : Q9NY61
Chromosome Location : 17q12
Pathway : Cell death signalling via NRAGE, NRIF and NADE, organism-specific biosystem; NRAGE signals death through JNK, organism-specific biosystem; Regulation of retinoblastoma protein, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signalling by NGF, organism-specific biosystem; p75 NTR receptor-mediated signalling, organism-specific biosystem;
Function : leucine zipper domain binding; protein binding; protein domain specific binding; sequence-specific DNA binding transcription factor activity; tau protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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