"COS-7" Related Products

    COS-7 (African green monkey kidney) whole cell lysate

    Cat.No. : COS-7-386M
    Species : African green monkey
    Tissue Type : Kidney; Sv40 Transformed
    Preparation method : Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml.
    Size : 200 ug
    Recommended Usage : Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
    Storage Buffer : In modified RIPA Lysis Buffer.
    Applications : Western Blot;

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