HCT 116 (human colorectal carcinoma) whole cell lysate

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HCT 116 (human colorectal carcinoma) whole cell lysate

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Cat.No. : HCT-776H
Species : Human
Tissue Type : Colon; Colorectal Carcinoma
Preparation method : Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml.
Size : 100 ul
Recommended Usage : Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer : In modified RIPA Lysis Buffer.
Applications : Western Blot;Immunoprecipitation;

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