- Mouse(1)
LADMAC (mouse bone marrow, producing colony stimulating factor-1) whole cell lysate
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Cat.No. : | LADMAC-966M |
Species : | Mouse |
Tissue Type : | Bone Marrow |
Preparation method : | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml. |
Size : | 200 ug |
Recommended Usage : | Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Storage Buffer : | In modified RIPA Lysis Buffer. |
Applications : | Western Blot;Immunoprecipitation; |
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