MOLT-4 (human acute lymphoblastic leukemia, T cell) whole cell lysate
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|Tissue Type :||T Cell Lymphoblast|
|Preparation method :||Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2.5 mg/ml, and then mixed with 5X Sample Buffer to become final 2 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.|
|Size :||200 ug|
|Recommended Usage :||The lysate is ready to load on SDS-PAGE for Western blotting.|
|Storage Buffer :||In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).|
|Applications :||Western Blot;|