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SW480 (Human colon adenocarcinoma) whole cell lysate

Cat.No. : SW480-1728H
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Species : Human
Tissue Type : Colon
Preparation method : Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml.
Size : 200 ug
Recommended Usage : Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer : In modified RIPA Lysis Buffer.
Applications : Western Blot;Immunoprecipitation;

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◆ Lysates
SW480-178H SW480 Whole Cell Lysate +Inquiry

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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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