SW480 (Human colon adenocarcinoma) whole cell lysate
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|Tissue Type :||Colon|
|Preparation method :||Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml.|
|Size :||200 ug|
|Recommended Usage :||Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.|
|Storage Buffer :||In modified RIPA Lysis Buffer.|
|Applications :||Western Blot;Immunoprecipitation;|