293T whole cell lysate
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|Tissue Type :||Kidney|
|Preparation method :||Culture cells in 6-well plate to 80% confluent, remove media and then rinse with cold 1x PBS. Scrape cells off the plate surface and pipette cells into microcentrifuge tube to spin down the cells. Remove PBS and then lysis and resuspend cells with 205 ul 1X sample buffer. Mix well and heat at 90°C for 10 minutes, and then cooled rapidly.|
|Size :||100 uL|
|Recommended Usage :||The lysate is ready to load on SDS-PAGE for Western blotting.|
|Storage Buffer :||In ready-to-use 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).|
|Applications :||Western Blot;|