| Cat.No.: | B16-F10-2107HCL |
| Species: | Mouse |
| Tissue Type: | Melanoma |
| Preparation method: | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml. |
| Size: | 100 ul |
| Recommended Usage: | Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
| Storage Buffer: | In modified RIPA Lysis Buffer. |
| Applications: | Western Blot;Immunoprecipitation; |
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