- Mouse(1)
B16-F10 (mouse skin melanoma) whole cell lysate
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Cat.No. : | B16-F10-2107HCL |
Species : | Mouse |
Tissue Type : | Melanoma |
Preparation method : | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml. |
Size : | 100 ul |
Recommended Usage : | Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Storage Buffer : | In modified RIPA Lysis Buffer. |
Applications : | Western Blot;Immunoprecipitation; |
B2MB4GALT1B3GAT1B3GALT2B3GAT3B3GNT2B4GALNT1B4GALT4B3GNT1B3GNT3B4GALT5B3GALT6B3GNT6B4GALT3B4GALT7B-LAB3GNT4B3GNT5B3GALNT1B3GALTLB3GAT2B4GALT6B4GALNT3B4GALT2B9D2B3GALNT2B3galt5B3GNTL1B4GALNT2B9D1B230208H17RikB020004J07RikB020031M17RikB230118H07RikB230120H23RikB230217C12RikB230219D22RikB230312A22RikB630005N14RikB930041F14RikB930007M17RikB3GALT4B4galnt4B430306N03RikB3gnt7B3gnt8B3gnt9
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