COLO 320HSR (human adenocarcinoma) whole cell lysate

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COLO 320HSR (human adenocarcinoma) whole cell lysate

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Cat.No. : COLO 320HSR-2145H
Species : Human
Tissue Type : Colon
Preparation method : Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2.5 mg/ml, and then mixed with 5X Sample Buffer to become final 2 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Size : 200 ug
Recommended Usage : The lysate is ready to load on SDS-PAGE for Western blotting.

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