HCT 116 (human colorectal carcinoma) whole cell lysate

• Cat.No.: HCT 116-2146H

Specification

• Species: Human
• Tag: Non
• Preparation method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2.5 mg/ml, and then mixed with 5X Sample Buffer to become final 2 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
• Size: 200 ug
• Recommended Usage: The lysate is ready to load on SDS-PAGE for Western blotting.