Recombinant Hepatitis C virus (HCV-1a) E2, His-tagged

• Cat.No.: HCV-1aE2-30H
• Product Overview: A DNA sequence encoding the Hepatitis C virus (serotype 1a, isolate H77) E2 protein (NP_751921.1) (Glu1-Glu278) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: HCV
• Source: Human Cells
• Tag: His
• Protein Length: 1-278 a.a.
• Description: E2 is a viral structural protein found in the hepatitis C virus. It is present on the viral membrane and functions as a host receptor binding protein, mediating entry into host cells. Therefore, it is a key target for the design of entry inhibitors and vaccine immunogens. Hepatitis C virus (HCV or sometimes HVC) is a small (55–65 nm in size), enveloped, positive-sense single-stranded RNA virus of the family Flaviviridae. Hepatitis C virus is the cause of hepatitis C in humans.
• Predicted N Terminal: Glu
• Form: Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.
• Molecular Mass: The recombinant virus E2 consists 289 amino acids and predicts a molecular mass of 32 kDa.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the lal
• Purity: >95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20? to -80?. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.