Recombinant Mouse Pla2g2e, His-tagged

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Recombinant Mouse Pla2g2e, His-tagged

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Cat.No. : Pla2g2e-1196M
Product Overview : A DNA sequence encoding the mouse PLA2G2E (Met 1-Cys 142) was expressed, with a C-terminal polyhistidine tag.
Description : Mouse Group IIE secretory phospholipase A2, also known as GIIE sPLA2, sPLA2-IIE, Phosphatidylcholine 2-acylhydrolase 2E and PLA2G2E is a secreted protein which belongs to the?phospholipase A2 family. Mammalian secretory phospholipase A2s ( sPLA2s ) form a family of structurally related enzymes that are involved in a variety of physiological and pathological processes via the release of arachidonic acid from membrane phospholipids or the binding to specific membrane receptors. Phospholipases A2 / PLA2 are enzymes that release fatty acids from the second carbon group of glycerol. This particular phospholipase specifically recognizes the sn-2 acyl bond of phospholipids and catalytically hydrolyzes the bond releasing arachidonic acid and lysophospholipids. Phospholipases A2 / PLA2 are commonly found in mammalian tissues as well as insect and snake venom. Venom from both snakes and insects is largely composed of melittin, which is a stimulant of Phospholipases A2 / PLA2. Due to the increased presence and activity of Phospholipases A2 / PLA2 resulting from a snake or insect bite, arachidonic acid is released from the phospholipid membrane disproportionately. As a result, inflammation and pain occur at the site. PLA2G2E catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a preference for arachidonic-containing phospholipids.
Source : Human Cells
Species : Mouse
Tag : His
Predicted N Terminal : Asn 20
Form : Lyophilized from sterile PBS, 10% glycerol, pH 7.4
Molecular Mass : The secreted recombinant mouse PLA2G2E comprises 134 amino acids and has a calculated molecular mass of 15.5 kDa. The apparent molecular mass of the recombinant protein is approximately 18 kDa in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 eu per μg of the protein as determined by the LAL method.
Purity : >97 % as determined by SDS-PAGE
Stability : Samples are stable for up to twelve months from date of receipt at -70℃
Storage : Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : A hardcopy of COA with reconstitution instruction is sent along with the products.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : Pla2g2e?phospholipase A2, group IIE [?Mus musculus?(house mouse) ]
Official Symbol : Pla2g2e
Synonyms : Pla2g2e; phospholipase A2, group IIE; group IIE secretory phospholipase A2; sPLA2-IIE; GIIE sPLA2; mGIIEsPLA2s; phosphatidylcholine 2-acylhydrolase 2E; NP_036174.1; EC 3.1.1.4
Gene ID : 26970
mRNA Refseq : NM_012044
Protein Refseq : NP_036174
UniProt ID : Q9QUL3
Chromosome Location : 4 D3; 4
Pathway : Acyl chain remodelling of PC; Arachidonic acid metabolism; Ether lipid metabolism
Function : calcium ion binding; hydrolase activity; metal ion binding; phospholipase A2 activity
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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