Recombinant Human BCHE, His-tagged

• Cat.No.: BCHE-10H
• Product Overview: Recombinant human BCHE (amino acids 29 – 568) with C-terminal His-tag was produced from the synthetic gene in Trichoplusia ni larval expression system.

Specification

• Description: BCHE is an esterase with broad substrate specificity. It contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
• Source: Trichoplusia ni larval expression system
• Species: Human
• Tag: His
• Form: 1 mg/mL in 50 mM Tris, 600 mM NaCl, 15% (w/v) glycerol pH 8.0, 0.2m filtered
• AA Sequence: DLEDDIIIAT KNGKVRGMNL TVFGGTVTAF LGIPYAQPPL GRLRFKKPQS LTKWSDIWNA TKYANSCCQN IDQSFPGFHG SEMWNPNTDL SEDCLYLNVW IPAPKPKNAT VLIWIYGGGFQTGTSSLHVY DGKFLARVER VIVVSMNYRV GALGFLALPG NPEAPGNMGL FDQQLALQWV QKNIAAFGGN PKSVTLFGES AGAASVSLHL LSPGSHSLFT RAILQSGSFN APWAVTSLYEARNRTLNLAK LTGCSRENET EIIKCLRNKD PQEILLNEAF VVPYGTPLSV NFGPTVDGDF LTDMPDILLE LGQFKKTQIL VGVNKDEGTA FLVYGAPGFS KDNNSIITRK EFQEGLKIFFPGVSEFGKES ILFHYTDWVD DQRPENYREA LGDVVGDYNF ICPALEFTKK FSEWGNNAFF YYFEHRSSKL PWPEWMGVMH GYEIEFVFGL PLERRDNYTK AEEILSRSIV KRWANFAKYGNPNETQNNST SWPVFKSTEQ KYLTLNTEST RIMTKLRAQQ CRFWTSFFPK VLEMTGNIDE AEGRGSHHHH HH
• Purity: >90% as determined by SDS-PAGE
• Storage: Store at -80°C or colder upon arrival. Freeze-thaw cycles of this product should be avoided.

Gene Information

• Gene Name: BCHE butyrylcholinesterase [ Homo sapiens ]
• Official Symbol: BCHE
• Synonyms: E1; CHE1; CHE2; cholinesterase; cholinesterase 1; choline esterase II; pseudocholinesterase; butyrylcholine esterase; cholinesterase (serum) 2; acylcholine acylhydrolase
• Gene ID: 590
• mRNA Refseq: NM_000055
• Protein Refseq: NP_000046
• MIM: 177400
• UniProt ID: P06276
• Chromosome Location: 3q26.1-q26.2
• Pathway: Glycerophospholipid biosynthesis, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Phospholipid metabolism, organism-specific biosystem
• Function: acetylcholinesterase activity; catalytic activity; enzyme binding