"CPB1" Related Products


Recombinant Pig Carboxypeptidase B

Cat.No.: CPB-5P
Description: Carboxypeptidase B, also well known as pancreatic procarboxy-peptidase B (PCPB), is a highly pancreas-specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitisand pancreatic graft rejection. As the prototype for those humanexopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preferencefor Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.
Species: Pig
Form: Lyophilized or Liquid
Molecular Mass: The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain.The secreted PCPB zymogen is converted to enzymatically active CPB by limited proteolysis by trypsin.
Unit Definition: One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH 7.65.
Enzyme Unit: ≥70 units per mg protein.
Storage: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.
Concentration: 1 -10 mg/mL
Storage Buffer: In 100 mM sodium chloride
Warning: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week.
Gene Name: CPB1 carboxypeptidase B1 (tissue) [ Sus scrofa(pig) ]
Official Symbol: CPB1
Synonyms: CPB1; CPB
Gene ID: 397341
mRNA Refseq: NM_214169
Protein Refseq: NP_999334
UniProt ID: P09955
Chromosome Location: 13; 13
Pathway: Pancreatic secretion, organism-specific biosystem; Protein digestion and absorption, organism-specific biosystem
Function: metallocarboxypeptidase activity; zinc ion binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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