"Imino-Biotin" Related Products

Imino-Biotin Beads

Cat.no: Imino-Biotin-12A
Product Overview: The Imino-Biotin Beads enable mild affinity purification of various proteins belonging to the Avidins family. The Imino-Biotin molecules immobilized on the beads are capable of binding Avidins at neutral and basic pH and eluting in under acidic conditions. The Imino-Biotin Beads can be regenerated at least 10 times with no significant loss in binding capacity.
Matrix: Highly cross-linked spherical agarose, 4%
Functional group: Imino-Biotin
Average particle size: 40-140 μm
Coupling chemistry: Epoxy
Dynamic binding capacity: 2-2.6 mg Avidin per ml of beads
Recommended flow rate: 1-2 ml/min/cm2
pH working range: 2-11
Elution buffer: 0.1 M acetic acid, pH 2.9

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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