Imino-Biotin Beads

• Cat.No.: Imino-Biotin-12A
• Product Overview: The Imino-Biotin Beads enable mild affinity purification of various proteins belonging to the Avidins family. The Imino-Biotin molecules immobilized on the beads are capable of binding Avidins at neutral and basic pH and eluting in under acidic conditions. The Imino-Biotin Beads can be regenerated at least 10 times with no significant loss in binding capacity.

Specification

• Tag: Non
• Cat.no: Imino-Biotin-12A
• Matrix: Highly cross-linked spherical agarose, 4%
• Functional group: Imino-Biotin
• Average particle size: 40-140 μm
• Coupling chemistry: Epoxy
• Dynamic binding capacity: 2-2.6 mg Avidin per ml of beads
• Recommended flow rate: 1-2 ml/min/cm2
• pH working range: 2-11
• Elution buffer: 0.1 M acetic acid, pH 2.9