"mono-avidin" Related Products

Mono-Avidin Beads

Cat.no: mono-avidin-13A
Product Overview: Our Mono-Avidin Beads enable mild affinity purification of biotinylated proteins, peptides and other ligands. The mono-avidin molecules immobilized on the beads have a much lower biotin-binding affinity than native avidin thus enabling dissociation of biotinylated molecules using free biotin molecules or low pH buffer. In addition, the Mono-Avidin Beads can be regenerated at least 10 times with no significant loss in binding capacity.
Matrix: Highly cross-linked spherical agarose, 4%
Functional group: Mono-Avidin
Average particle size: 40-140 μm
Coupling chemistry: Epoxy
Dynamic binding capacity: 2-2.6 mg Avidin per ml of beads
Recommended flow rate: 1-2 ml/min/cm2
Elution buffer: 2 mM D-biotin in Phosphate buffer saline

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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