|Product Overview:||Our Mono-Avidin Beads enable mild affinity purification of biotinylated proteins, peptides and other ligands. The mono-avidin molecules immobilized on the beads have a much lower biotin-binding affinity than native avidin thus enabling dissociation of biotinylated molecules using free biotin molecules or low pH buffer. In addition, the Mono-Avidin Beads can be regenerated at least 10 times with no significant loss in binding capacity.|
|Matrix:||Highly cross-linked spherical agarose, 4%|
|Average particle size:||40-140 μm|
|Dynamic binding capacity:||2-2.6 mg Avidin per ml of beads|
|Recommended flow rate:||1-2 ml/min/cm2|
|Elution buffer:||2 mM D-biotin in Phosphate buffer saline|
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