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Active Recombinant Mouse Fcgr1 protein(Met1-Pro297), His&Avi-tagged, Biotinylated

Cat.No. : Fcgr1-190M
Product Overview : Biotinylated Recombinant Mouse FCGR1 (NP_034316.1)(Met 1-Pro 297) was was expressed in HEK293, fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.
  • Specification
  • Gene Information
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Source : HEK293
Species : Mouse
Tag : C-His&Avi
Protein length : Met1-Pro297
Conjugation/Label : Biotin
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : 1. Immobilized Rituximab (IgG1) at 1 μg/mL can bind Biotinylated Mouse FcγRI / CD64 recombinant protein (His & Avi Tag), the EC50 is 20-50 ng/mL. 2. Loaded Biotinylated Mouse FcγRI / CD64 recombinant protein (His & Avi Tag) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.66 μM as determined in a BLI assay (ForteBio Octet Red384). 3. Loaded Biotinylated Mouse FcγRI / CD64 recombinant protein (His & Avi Tag) on SA Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 1.9 μM as determined in a BLI assay (ForteBio Octet Red384).
Molecular Mass : The recombinant mouse FCGR1 consists of 308 amino acids after removal of the signal peptide and has a predicted molecular mass of 34.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR1 is approximately 50-55 kDa due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Fcgr1 Fc receptor, IgG, high affinity I [ Mus musculus ]
Official Symbol : Fcgr1
Synonyms : FCGR1; Fc receptor, IgG, high affinity I; high affinity immunoglobulin gamma Fc receptor I; fcRI; fc-gamma RI; igG Fc receptor I; IgG high affinity Fc receptor; CD64; IGGHAFC; AI323638; AV092959; FcgammaRI;
Gene ID : 14129
mRNA Refseq : NM_010186
Protein Refseq : NP_034316

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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