Native Streptomyces sp. Phospholipase D, Type VII

Cat.No.: PLD-19S
Product Overview: Native Phospholipase D, Type VII, was purified from Streptomyces sp. CAS#9001-87-0
Description: Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. Phospholipase D is glycerophospholipid-specific. It is markedly less active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Source: Streptomyces sp.
Species: Streptomyces sp.
Form: lyophilized powder. Bottomless glass bottle. Contents are inside inserted fused cone.
Bio-activity: ≥150 units/mg solid
Unit Definition: One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.
Applications: Phospholipase D (PLD) has been used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to yield the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD has been used in purification and kinetic studies. The enzyme has been used in the translocation of sphingosine kinase 1 (SK1) to membrane fractions under in vitro conditions. It has also been used to produce phosphatidic acid (PA) from phosphatidylcholine (PC) in HL60 permeabilized cells.
Notes: Protein by biuret
Storage: Store at -20 °C

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