Active Native Streptomyces sp. Phospholipase D, Type VII
Cat.No. : |
PLD-19S |
Product Overview : |
Native Phospholipase D, Type VII, was purified from Streptomyces sp. CAS#9001-87-0 |
- Specification
- Gene Information
- Related Products
Description : |
Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. Phospholipase D is glycerophospholipid-specific. It is markedly less active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. |
Source : |
Streptomyces sp. |
Species : |
Streptomyces sp. |
Form : |
lyophilized powder. Bottomless glass bottle. Contents are inside inserted fused cone. |
Bio-activity : |
≥150 units/mg solid |
Unit Definition : |
One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C. |
Applications : |
Phospholipase D (PLD) has been used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to yield the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD has been used in purification and kinetic studies. The enzyme has been used in the translocation of sphingosine kinase 1 (SK1) to membrane fractions under in vitro conditions. It has also been used to produce phosphatidic acid (PA) from phosphatidylcholine (PC) in HL60 permeabilized cells. |
Notes : |
Protein by biuret |
Storage : |
Store at -20 °C |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.