Active Recombinant Human KDM2B, FLAG-tagged

• Cat.No.: KDM2B-40H
• Product Overview: Recombinant Human KDM2B was expressed in Sf9 cells and contains an N-terminal FLAG tag.

Specification

• Description: KDM2B (lysine (K)-specific demethylase 2B), also known as FBXL10 (F-box and leucine-rich repeat protein 10) is a histone demethylase that preferentially demethylates trimethylated lysine 4 (K4me3) and dimethylated lysine 36 (K36me2) of histone H3. KDM2B displays weak or no activity for mono- and trimethylated H3K36. KDM2B preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which results in inhibition of cell growth and proliferation. KDM2B may also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
• Source: Sf9 insect cells
• Species: Human
• Tag: FLAG
• Form: 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol.
• Bio-activity: H3K36me2 demethylase.
• Molecular Mass: 155.9 kDa
• Applications: Binding assays, inhibitor screening, and selectivity profiling.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.8 mg/ml

Gene Information

• Gene Name: KDM2B lysine (K)-specific demethylase 2B [ Homo sapiens ]
• Official Symbol: KDM2B
• Synonyms: KDM2B; lysine (K)-specific demethylase 2B; F box and leucine rich repeat protein 10 , FBXL10; lysine-specific demethylase 2B; CXXC2; Fbl10; JHDM1B; jumonji C domain containing histone demethylase 1B; PCCX2; F-box protein FBL10; F-box/LRR-repeat protein 10; CXXC-type zinc finger protein 2; protein containing CXXC domain 2; protein-containing CXXC domain 2; [Histone-H3]-lysine-36 demethylase 1B; F-box and leucine-rich repeat protein 10; jumonji C domain-containing histone demethylase 1B; jmjC domain-containing histone demethylation protein 1B; JEMMA (Jumonji domain, EMSY-interactor, methyltransferase motif) protein; jumonji domain-containing EMSY-interactor methyltransferase motif protein; FBXL10
• Gene ID: 84678
• mRNA Refseq: NM_001005366
• Protein Refseq: NP_001005366
• MIM: 609078
• UniProt ID: Q8NHM5
• Chromosome Location: 12q24.31
• Function: DNA binding; DNA binding; histone demethylase activity; histone demethylase activity (H3-K36 specific); metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding; rRNA binding; zinc ion binding; zinc ion binding