||Recombinant Human PHIP corresponding to amino acids 1302 - 1434 that contains the bromodomain sequences of PHIP (accession number NP_060404.3) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 21.994 kDa.
||PH-interacting protein (PHIP), also known as WDR11 or BRWD2, is a member of the WD repeat protein family. This family is characterized by regions of approximately 40 amino acids bracketed by gly-his and trp-asp (GH-WD), that potentially facilitate the formation of heterotrimeric or multimeric protein complexes. They also contain bromodomains that recognize acetylated histone lysine residues and function as "readers" of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
||25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol.
||Binding assays, inhibitor screening, and selectivity profiling.
||Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.