Recombinant Human TRIM33, His&FLAG-tagged

• Cat.No.: TRIM33-83H
• Product Overview: Recombinant Human TRIM33 corresponding to amino acids 959 - 1059 that contains the bromodomain sequences of TRIM33 (accession number NP_056990.3) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 17.7 kDa.

Specification

• Description: Tripartite motif-containing 33 (TRIM33) protein, also known as TIF1γ, is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes TRIM24 (TIF1α), TRIM28 (TIF1β) and TRIM33 (TIF1γ) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as "readers" of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
• Source: E. coli
• Species: Human
• Tag: His&FLAG
• Form: 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol.
• Applications: Binding assays, inhibitor screening, and selectivity profiling.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: TRIM33 tripartite motif containing 33 [ Homo sapiens ]
• Official Symbol: TRIM33
• Synonyms: TRIM33; tripartite motif containing 33; E3 ubiquitin-protein ligase TRIM33; FLJ11429; KIAA1113; PTC7; ret fused gene 7; RFG7; TF1G; TIF1G; TIF1GAMMA; TIFGAMMA; transcriptional intermediary factor 1 gamma; TIF1-gamma; protein Rfg7; ectodermin homolog; RET-fused gene 7 protein; tripartite motif-containing 33; ECTO; FLJ32925
• Gene ID: 51592
• mRNA Refseq: NM_015906
• Protein Refseq: NP_056990
• MIM: 605769
• UniProt ID: Q9UPN9
• Chromosome Location: 1p13.1
• Function: DNA binding; R-SMAD binding; co-SMAD binding; ligase activity; metal ion binding; protein binding; zinc ion binding