|Cat. No. :
|Product Overview :
||The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity. Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.
|Physical Appearance :
||Sterile Filtered clear colorless solution (11.1 mg/ml).
||95% pure by Gel Filtration.
||50% glycerol, 5 mM MgCl2, 0.1 mM ZnCl2 and 5 mM TRIS, pH 7.0.
|Biological Activity :
||>1500 U/mg (pH 9.6, 37°C and 0.025 M glycin, 10% glycerol as buffer).
||AP although stable at 14°C for 1 weeks, should be stored at 4°C. Please Do-Not freeze.
||EC 184.108.40.206; IAP; AP; Alk_phosphatase; glycerophosphatase; Kasahara isozyme; alkaline phosphatase, intestinal; alkaline phosphomonoesterase; intestinal alkaline phosphatase; alkaline phosphatase; alkaline phosphomonoesterase; phosphomonoesterase; glycerophosphatase; alkaline phosphohydrolase; alkaline phenyl phosphatase; orthophosphoric-monoester phosphohydrolase (alkaline optimum); phosphate-monoester phosphohydrolase (alkaline optimum).