Bovine Intestinal Alkaline Phosphatase BIAP-74B

Bovine Intestinal Alkaline Phosphatase

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Bovine Intestinal Alkaline Phosphatase

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Cat. No. : BIAP-74B
Product Overview : The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity. Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.
Source : Calf Intestine.
Physical Appearance : Sterile Filtered clear colorless solution (11.1 mg/ml).
Purity : 95% pure by Gel Filtration.
Formulation : 50% glycerol, 5 mM MgCl2, 0.1 mM ZnCl2 and 5 mM TRIS, pH 7.0.
Biological Activity : >1500 U/mg (pH 9.6, 37°C and 0.025 M glycin, 10% glycerol as buffer).
Stability : AP although stable at 14°C for 1 weeks, should be stored at 4°C. Please Do-Not freeze.
Synonyms : EC 3.1.3.1; IAP; AP; Alk_phosphatase; glycerophosphatase; Kasahara isozyme; alkaline phosphatase, intestinal; alkaline phosphomonoesterase; intestinal alkaline phosphatase; alkaline phosphatase; alkaline phosphomonoesterase; phosphomonoesterase; glycerophosphatase; alkaline phosphohydrolase; alkaline phenyl phosphatase; orthophosphoric-monoester phosphohydrolase (alkaline optimum); phosphate-monoester phosphohydrolase (alkaline optimum).

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