Recombinant E.coli LacI, His-tagged

• Cat.No.: lacI-89E
• Product Overview: Recombinant E.coli Lactose operon repressor/LacI is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Gln360) of E.coli LacI fused with a 6His tag at the C-terminus.

Specification

• Description: Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
• Source: E. coli
• Species: E. coli
• Tag: His
• Form: Supplied as a 0.2 μM filtered solution of 20mM Tris, 300mM NaCl, 5mM DTT pH 8.0
• AA Sequence: MKPVTLYDVAEYAGVSYQTVSRVVN QASHVSAKTREKVEAAMAELNYIPN RVAQQLAGKQSLLIG VATSSLALHAPSQIVAAIKSRADQL GASVVVSMVERSGVEACKAAVHNLL AQRVSGLIINYPLDD QDAIAVEAACTNVPALFLDVSDQTP INSIIFSHEDGTRLGVEHLVALGHQ QIALLAGPLSSVSAR LRLAGWHKYLTRNQIQPIAEREGDW SAMSGFQQTMQMLNEGIVPTAMLVA NDQMALGAMRAITES GLRVGADISVVGYDDTEDSSCYIPP LTTIKQDFRLLGQTSVDRLLQLSQG QAVKGNQLLPVSLVK RKTTLAPNTQTASPRALADSLMQLA RQVSRLESGQHHHHHH
• Endotoxin: Less than 0.1 ng/μg (1 IEU/μg).
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Storage: Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.