Recombinant Human Estrogen beta receptor LBD/Hsp90 complex, GST-tagged

• Cat.No.: ESR2-103H
• Product Overview: Recombinant Human GST-tagged Estrogen beta receptor ligand-binding domain (ERβ-LBD) consists of a 218 a.a. GST tag, a 30 a.a. flexible linker and a 346 a.a. ERβ-LBD, Acc# NP_001428, amino acids 243-530. It was produced in complex with human Hsp90 to facilitate native protein conformation and advanced performance in ligand binding and coactivator peptide recruitment assays. GST tagged ERβ-LBD and human Hsp90 are major components of the complex. Identity of ERβ-LBD and Hsp90 in the complex was confirmed using mass spectrometry.

Specification

• Source: Sf9 insect cells
• Species: Human
• Tag: GST
• Form: 40 mM Na-P pH 8.0, 40 mM KCl, 0.02% NP40, 7 mM reduced glutathione, 20mM Na2MoO4, 15% glycerol.
• Purity: >90%
• Applications: Ligand binding and coactivator peptide recruitment assays.
• Storage: Immediately store at -80℃. Avoid freeze-thaw cycles.While working, please keep sample on ice.

Gene Information

• Gene Name: ESR2 estrogen receptor 2 (ER beta) [ Homo sapiens ]
• Official Symbol: ESR2
• Synonyms: ESR2; estrogen receptor 2 (ER beta); estrogen receptor beta; Erb; NR3A2; estrogen receptor beta 4; estrogen nuclear receptor beta variant a; estrogen nuclear receptor beta variant b; nuclear receptor subfamily 3 group A member 2; ESRB; ESTRB; ER-BETA; ESR-BETA
• Gene ID: 2100
• mRNA Refseq: NM_001040275
• Protein Refseq: NP_001035365
• MIM: 601663
• UniProt ID: Q92731
• Chromosome Location: 14q21-q22
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem; Nuclear Receptors, organism-specific biosystem; Ovarian Infertility Genes, organism-specific biosystem; Plasma membrane estrogen receptor signaling, organism-specific biosystem
• Function: DNA binding; core promoter sequence-specific DNA binding; enzyme binding; estrogen receptor activity; estrogen response element binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; metal ion binding; protein binding; receptor activity; receptor antagonist activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid binding; steroid hormone receptor activity; transcription coactivator activity; zinc ion binding