Recombinant Chicken Lactate Dehydrogenase B LDHB-82C

Recombinant Chicken Lactate Dehydrogenase B

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Recombinant Chicken Lactate Dehydrogenase B

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Cat. No. : LDHB-82C
Product Overview : Recombinant chicken LDH-B produced inE.Coliis cloned from cDNA library of chicken heart.
Description : Lactate dehydrogenase (LDH) is an enzyme (EC 1.1.1.27) present in a wide variety of organisms, including plants and animals. A tetrameric enzyme that catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
Source : Escherichia Coli.
Physical Appearance : Sterile lyophilized powder.
Purity : Greater than 95.0% as determined by: (a) Analysis by RP-HPLC (b) Analysis by SDS-PAGE.
Formulation : The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.
Biological Activity : The specific activity was found to be 200 IU/mg.
Unit Definition : One unit is defined as 1 μmol of NAD+ production per minute under the assay conditions (25°C, pH 7.0). Both transaminase activities include α-hydroxyglutarate dehydrogenase activity.
Stability : Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Gene Name : LDHB lactate dehydrogenase B [ Gallus gallus ]
Synonyms : LDHB; lactate dehydrogenase B; L-lactate dehydrogenase B; EC 1.1.1.27
Gene ID : 373997
mRNA Refseq : NM_204177
Protein Refseq : NP_989508
UniProt ID : P00337
Chromosome Location : 1
Pathway : Cysteine and methionine metabolism; Glycolysis/Gluconeogenesis; Metabolic pathways; Propanoate metabolism; Pyruvate metabolism
Function : L-lactate dehydrogenase activity; binding; oxidoreductase activity

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