|Cat. No. :
|Product Overview :
||Recombinant chicken LDH-B produced inE.Coliis cloned from cDNA library of chicken heart.
||Lactate dehydrogenase (LDH) is an enzyme (EC 22.214.171.124) present in a wide variety of organisms, including plants and animals. A tetrameric enzyme that catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
|Physical Appearance :
||Sterile lyophilized powder.
||Greater than 95.0% as determined by: (a) Analysis by RP-HPLC (b) Analysis by SDS-PAGE.
||The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.
|Biological Activity :
||The specific activity was found to be 200 IU/mg.
|Unit Definition :
||One unit is defined as 1 μmol of NAD+ production per minute under the assay conditions (25°C, pH 7.0). Both transaminase activities include α-hydroxyglutarate dehydrogenase activity.
||Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.