Cat. No. :	LDHB-82C
Product Overview :	Recombinant chicken LDH-B produced inE.Coliis cloned from cDNA library of chicken heart.
Description :	Lactate dehydrogenase (LDH) is an enzyme (EC 1.1.1.27) present in a wide variety of organisms, including plants and animals. A tetrameric enzyme that catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
Source :	Escherichia Coli.
Physical Appearance :	Sterile lyophilized powder.
Purity :	Greater than 95.0% as determined by: (a) Analysis by RP-HPLC (b) Analysis by SDS-PAGE.
Formulation :	The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.
Biological Activity :	The specific activity was found to be 200 IU/mg.
Unit Definition :	One unit is defined as 1 μmol of NAD+ production per minute under the assay conditions (25°C, pH 7.0). Both transaminase activities include α-hydroxyglutarate dehydrogenase activity.
Stability :	Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

Gene Name :	LDHB lactate dehydrogenase B [ Gallus gallus ]
Synonyms :	LDHB; lactate dehydrogenase B; L-lactate dehydrogenase B; EC 1.1.1.27
Gene ID :	373997
mRNA Refseq :	NM_204177
Protein Refseq :	NP_989508
UniProt ID :	P00337
Chromosome Location :	1
Pathway :	Cysteine and methionine metabolism; Glycolysis/Gluconeogenesis; Metabolic pathways; Propanoate metabolism; Pyruvate metabolism
Function :	L-lactate dehydrogenase activity; binding; oxidoreductase activity

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