Recombinant Chicken Lactate Dehydrogenase B LDHB-82C

Recombinant Chicken Lactate Dehydrogenase B


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Recombinant Chicken Lactate Dehydrogenase B

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Cat. No. : LDHB-82C
Product Overview : Recombinant chicken LDH-B produced inE.Coliis cloned from cDNA library of chicken heart.
Description : Lactate dehydrogenase (LDH) is an enzyme (EC present in a wide variety of organisms, including plants and animals. A tetrameric enzyme that catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
Source : Escherichia Coli.
Physical Appearance : Sterile lyophilized powder.
Purity : Greater than 95.0% as determined by: (a) Analysis by RP-HPLC (b) Analysis by SDS-PAGE.
Formulation : The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.
Biological Activity : The specific activity was found to be 200 IU/mg.
Unit Definition : One unit is defined as 1 μmol of NAD+ production per minute under the assay conditions (25°C, pH 7.0). Both transaminase activities include α-hydroxyglutarate dehydrogenase activity.
Stability : Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Gene Name : LDHB lactate dehydrogenase B [ Gallus gallus ]
Synonyms : LDHB; lactate dehydrogenase B; L-lactate dehydrogenase B; EC
Gene ID : 373997
mRNA Refseq : NM_204177
Protein Refseq : NP_989508
UniProt ID : P00337
Chromosome Location : 1
Pathway : Cysteine and methionine metabolism; Glycolysis/Gluconeogenesis; Metabolic pathways; Propanoate metabolism; Pyruvate metabolism
Function : L-lactate dehydrogenase activity; binding; oxidoreductase activity
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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