Active Recombinant Human VCPIP, GST-tagged

• Cat.No.: VCPIP-129H
• Product Overview: Recombinant Human VCPIP CD(25-561), fused with GST tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 25-561 a.a.
• Description: VCPIP is a cysteine protease and is a member of the OTU superfamily of proteins. Cloning of the human gene was first described by Nagase et al.
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylase Enzyme Assay: The activity of GST-VCPIP was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-VCPIP was compared confirming the deubiquitylating activity of GST-VCPIP.
• Molecular Mass: ~87kDa
• Storage: 12 months at -70℃; aliquot as required.
• Concentration: 0.5 mg/ml