"VCPIP" Related Products

Recombinant Human VCPIP, GST-tagged

Cat.No.: VCPIP-129H
Product Overview: Recombinant Human VCPIP CD(25-561), fused with GST tag, was expressed in E.coli.
Description: VCPIP is a cysteine protease and is a member of the OTU superfamily of proteins. Cloning of the human gene was first described by Nagase et al.
Source: E. coli
Species: Human
Tag: GST
Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity: Deubiquitylase Enzyme Assay: The activity of GST-VCPIP was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-VCPIP was compared confirming the deubiquitylating activity of GST-VCPIP.
Molecular Mass: ~87kDa
Storage: 12 months at -70?C; aliquot as required.
Concentration: 0.5 mg/ml

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