Active Recombinant Human CUL3/RBX1

• Cat.No.: CUL3-148H
• Product Overview: Recombinant human CUL3 (amino acid residues 1-768), RBX1 (amino acid residues 1-108) was expressed in Insect (Sf21) cells.

Specification

• Description: Cullin-RING-Ligases (CRLs) are one largest class of ubiquitin E3 ligases and the enzymes of the NEDDylation pathway play a pivotal role in the activation of these, akin to ubiquitylation, the E1 activating enzyme (APP-BP1/UBA3 heterodimer) and the E2 conjugating enzymes (UBE2M or UBE2F) are involved in mammalian NEDDylation of the Cullin Ring Ligases (CRLs) (Meyer-Schaller et al., 2009; Huang et al., 2011; Morimoto et al., 2003).
• Source: Insect cells
• Species: Human
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: E3 Ligase Assay: The activity of Cul3/Rbx1 was validated indirectly through its ability to act as a substrate for Neddylation in the presence of the NEDD8 E3 ligase His-DCNL2 and thioester-loaded His-Ube2M-NEDD8. Incubation of Cul3/Rbx1 and thioester loaded His-Ube2M-NEDD8 in the presence or absence of His-DCNL2 at 4?C was compared at two time points T0 and T2 minutes. Neddylation of the Cul3 subunit in the presence of His-DCNL2 was demonstrated.
• Molecular Mass: Cul3= 89kDa; Rbx1=12kDa
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: CUL3 cullin 3 [ Homo sapiens ]
• Official Symbol: CUL3
• Synonyms: CUL3; cullin 3; cullin-3; CUL-3; PHA2E; FLJ25665
• Gene ID: 8452
• mRNA Refseq: NM_001257197
• Protein Refseq: NP_001244126
• MIM: 603136
• UniProt ID: Q13618
• Chromosome Location: 2q36.2
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Aurora B signaling, organism-specific biosystem; Canonical Wnt signaling pathway, organism-specific biosystem; Class I MHC mediated antigen processing &
• Function: cyclin binding; protein binding; ubiquitin protein ligase binding; contributes_to ubiquitin-protein ligase activity