"USP27X" Related Products

Recombinant Human USP27X, DAC-tagged

Cat.No.: USP27X-155H
Product Overview: Recombinant human USP27X (amino acid residues 1-438), fused with N-terminal DAC, was expressed in E. coli.
Description: Deconjugating enzymes (DCEs) are proteases that process ubiquitin or ubiquitin-like gene products, reverse the modification of proteins by a single ubiquitin or ubiquitin-like protein (UBL) and remodel polyubiquitin (or poly-UBL) chains on target proteins (Reyes-Turcu et al., 2009). The deubiquitylating – or deubiquitinating – enzymes (DUBs) represent the largest family of DCEs and regulate ubiquitin dependent signalling pathways.
Source: E. coli
Species: Human
Tag: DAC
Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity: Deubiquitylase Enzyme Assay: The activity of DAC-USP27X was validated by the monitoring of mono-ubiquitin generation as a result of the enzyme catalysed cleavage of K63-linked di-ubiquitin. Incubation of the substrate in the presence or absence of DAC-USP27X was compared confirming the deubiquitylating activity of DAC-USP27X.
Molecular Mass: ~79.3kDa
Purity: >67% by SDS-PAGE
Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
Concentration: 0.5 mg/ml

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