Active Recombinant Human NEDD8
Cat.No. : | NEDD8-160H |
Product Overview : | Recombinant human NEDD8 (amino acid residues 1-76) was expressed in E. coli. |
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- Gene Information
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Description : | Neural Precursor Cell Expressed, Developmentally Downregulated 8 (NEDD8) is a member of the ubiquitin like modifiers and the human gene was first described by (Kamitani, et al., 1997). The heterodimeric E1, APP-BP1/UBA3 forms a complex in vitro and a thioester linkage with NEDD8 (Osaka et al., 1998). NEDD8 has also been co-crystallised with APP-BP1 and ATP (Walden et al., 2003). |
Source : | E. coli |
Species : | Human |
Form : | 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol |
Bio-activity : | E2 thioester NEDD8 loading assay. The activity of NEDD8 was validated by loading E1 APP-BP1/Uba3 activated NEDD8 onto the active cysteine of the His-UBE2M E2 enzyme via a transthiolation reaction. Incubation of the APP-BP1/Uba3 and His-UBE2M enzymes in the presence of NEDD8 and ATP at 30?C was compared at two time points, T0 and T10 minutes. Sensitivity of the NEDD8/His-UBE2M thioester bond to the reducing agent DTT was confirmed. |
Molecular Mass : | ~8.6kDa |
Purity : | >98% by SDS-PAGE |
Storage : | 12 months at -70°C. Avoid multiple freeze/thaw cycles. |
Concentration : | 1 mg/ml |
Gene Name : | NEDD8 neural precursor cell expressed, developmentally down-regulated 8 [ Homo sapiens ] |
Official Symbol : | NEDD8 |
Synonyms : | NEDD8; neural precursor cell expressed, developmentally down-regulated 8; Nedd 8; neddylin; ubiquitin-like protein Nedd8; NEDD-8; FLJ43224; MGC104393; MGC125896; MGC125897; |
Gene ID : | 4738 |
mRNA Refseq : | NM_006156 |
Protein Refseq : | NP_006147 |
MIM : | 603171 |
UniProt ID : | Q15843 |
Chromosome Location : | 14q11.2 |
Pathway : | p53 pathway, organism-specific biosystem; |
Function : | ubiquitin protein ligase binding; |
Products Types
◆ Recombinant Protein | ||
NEDD8-3609R | Recombinant Rat NEDD8 Protein, His (Fc)-Avi-tagged | +Inquiry |
NEDD8-6002M | Recombinant Mouse NEDD8 Protein, His (Fc)-Avi-tagged | +Inquiry |
NEDD8-2814R | Recombinant Rhesus Macaque NEDD8 Protein, His (Fc)-Avi-tagged | +Inquiry |
NEDD8-2018H | Recombinant Human NEDD8 Protein, His-tagged | +Inquiry |
NEDD8-29619TH | Recombinant Human NEDD8, His-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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