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Recombinant Human USP1/UAF1 Complex Protein, His-Tagged

Cat.No. : USP1-161H
Product Overview : Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP1/UAF1 Complex protein. Contains a C-terminal 6-His tag
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Description : USP1 (Ubiquitin-Specific-processing Protease 1, also known as Ubiquitin carboxyl-terminal hydrolase 1) is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the Fanconi Anemia pathway. Reported substrates of USP1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA. USP1 plays important roles in DNA damage responses and cancer-related processes, and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy. By itself, USP1 is nearly completely inactive and requires a protein binding partner, UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity. In addition to the naturally occurring substates listed above, the USP1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains. Recombinant USP1 protein contains a C-terminal 6-His tag, and glycine-to-alanine substitutions at positions 670 and 671 (UniProt O94782 numbering). Recombinant UAF1 protein also contains a C-terminal 6-His tag. The assembled complex contains 25 µg each of USP1 and UAF1, resulting in a 1:1.2 stoichiometry.
Source : Sf21 insect cells
Species : Human
Tag : His
Molecular Mass : 89 kDa (USP1), 77 kDa (UAF1)
Purity : >80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Applications : Bioactivity
Usage : Recombinant Human USP1/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1/UAF1 complex concentration of 0.01-0.5 μM.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 °C as supplied.
3 months, -70 °C under sterile conditions after opening.
Storage Buffer : X mg/ml total protein including USP1 (X µM) and UAF1(X µM) in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM TCEP
Shipping : The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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