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Recombinant Human USP1/UAF1, His-tagged

Cat.No.: USP1-161H
Product Overview: Recombinant human USP1 [6His-tagged] / UAF1 [untagged] heterodimer were co-expressed using an insect cell.
Description: There are two main classes of DUB, cysteine proteases and metalloproteases. Ubiquitin specific protease 1 (USP1) is a member of the cysteine protease enzyme family and cloning of the human gene was first described by Fujiwara et al. (1998). USP1 has been shown to act as a negative regulator of the Fanconi anaemia pathway. Inhibition of USP1 by siRNA knockdown increased the monoubiquitylation of the Fanconi anaemia effector protein, FANCD2, and increased cellular resistance to DNA cross-linking agents, suggesting that USP1 deubiquitylates FANCD2 (Huang et al., 2006).
Source: Insect cells
Species: Human
Tag: His
Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity: Deubiquitylase Enzyme Assay: The activity of 6His-USP1/UAF1 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of 6His-USP1/UAF1 was compared confirming the deubiquitylating activity of 6His-USP1/UAF1.
Molecular Mass: USP1 = 90.5 kDa/UAF1 = 76.2 kDa
Purity: >90% by SDS-PAGE
Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
Concentration: 0.5 mg/ml
Gene Name: USP1 ubiquitin specific peptidase 1 [ Homo sapiens ]
Official Symbol: USP1
Synonyms: USP1; ubiquitin specific peptidase 1; ubiquitin specific protease 1; ubiquitin carboxyl-terminal hydrolase 1; hUBP; ubiquitin thioesterase 1; deubiquitinating enzyme 1; ubiquitin thiolesterase 1; ubiquitin carboxyl terminal hydrolase 1; ubiquitin specific processing protease 1; ubiquitin-specific-processing protease 1; UBP;
Gene ID: 7398
mRNA Refseq: NM_001017415
Protein Refseq: NP_001017415
MIM: 603478
UniProt ID: O94782
Chromosome Location: 1p31.3
Pathway: DNA Repair, organism-specific biosystem; Fanconi Anemia pathway, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Regulation of the Fanconi anemia pathway, organism-specific biosystem;
Function: cysteine-type endopeptidase activity; peptidase activity; protein binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity; ubiquitin-specific protease activity;

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