Recombinant Human OTUD6A, His-tagged OTUD6A-166H

Recombinant Human OTUD6A, His-tagged

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Recombinant Human OTUD6A, His-tagged

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Cat.No. : OTUD6A-166H
Product Overview : Recombinant human OTUD6A (amino acid residues 1-288), fused with N-terminal His, was expressed in E.coli.
Description : OTUD6A is a cysteine protease and is a member of the OTU (ovarian tumour) superfamily of proteins (Balakirev et al., 2003). Cloning of the human gene was first described by Kayagaki et al. (2007). OTU enzymes play important roles as negative-feedback regulators in NF-κB signalling, interferon signalling and in p97 (cdc48)-mediated processes although the cellular functions of most OTU enzymes remain to be discovered.
Source : E. coli
Species : Human
Tag : His
Form : 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity : Deubiquitylase Enzyme Assay: The activity of 6His-OTUD6A was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of 6His-OTUD6A was compared confirming the deubiquitylating activity of 6His-OTUD6A.
Molecular Mass : 35.8 kDa
Purity : >98% by SDS-PAGE
Storage : 12 months at -70°C. Avoid multiple freeze/thaw cycles.
Concentration : 0.5 mg/ml
Gene Name : OTUD6A OTU domain containing 6A [ Homo sapiens ]
Official Symbol : OTUD6A
Synonyms : OTUD6A; OTU domain containing 6A; OTU domain-containing protein 6A; DUBA2; FLJ25831; HSHIN6; DUBA-2; HIN-6 protease;
Gene ID : 139562
mRNA Refseq : NM_207320
Protein Refseq : NP_997203
MIM : 300714
UniProt ID : Q7L8S5
Chromosome Location : Xq13.1

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